Association of variable number of tandem repeats (VNTR) and T941G polymorphism of monoamine oxidase (MAO-A) gene with aggression in Pakistani subjects.

Sumbal Sarwar,Shabana Shabana,Shahida Hasnain

doi:10.4314/ahs.v21i1.24

Sumbal Sarwar, Shabana Shabana + Show 1 more

Open Access

https://doi.org/10.4314/ahs.v21i1.24

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Journal: African health sciences	Publication Date: Mar 1, 2021
Citations: 2	License type: cc-by

Affiliation: University of the Punjab

Abstract

Human behavioral traits are known to be significantly heritable. Certain individuals have a greater tendency of negative behavioral aspects including aggression. The quest to identify tunderlying genetic causes has led to identification of a number of genetic markers, one of them is the monoamine oxidase-A (MAO-A) gene. We aimed to genotype a variable number of tandem repeats (VNTRs) in the promoter region and a functional SNP within this gene (T941G, dbSNP ID: rs6323) in the recruited cohort of 482 subjects. After DNA isolation, genotyping was done by PCR-RFLP and the results were confirmed by sequencing. For VNTRs, the results showed, highest frequency of 3.5 repeats in males and 4 repeats in females in the promoter region. The genotype frequencies for the SNP in cases were GG=16.3%, TG=20.6% and TT=63.1%, while in controls, the frequencies were GG=12.7%, TG=6.3%, and TT=81.0%. The allele frequencies were significantly different between cases and controls (p=0.015; OR=1.51; CI=1.085-2.102). The selected VNTR and SNP appeared to be significantly associated with aggression. These VNTRs and SNP have not been studied previously in the Pakistani population, hence they represent a unique ethnic group. These results, however, would have to be replicated in larger cohorts.

Highlights

Human behavior is a collection of various emotions, instincts and characteristics that may have positive or negative impact on the bearer and on other individuals of the society 1,2
The way 8) of monoamine oxidase A gene especially among we look and behave is determined by the complex inter- Asian population23, . 36-39 An initial analysis of aggressive action of hundreds of complex traits, each of which is samples revealed a greater number of males (60%) than in turn governed by a combination of inherited factors, females (40%)
Our results revealed that the polymorphism is significantly associated with aggression in local population (p=0.015)

Summary

Introduction

Human behavior is a collection of various emotions, instincts and characteristics that may have positive or negative impact on the bearer and on other individuals of the society 1,2. Aggression is one of the most important traits of human behavior It is a series of deliberate actions that leads to anguish, destruction or injury to other organisms. Objective: We aimed to genotype a variable number of tandem repeats (VNTRs) in the promoter region and a functional SNP within this gene (T941G, dbSNP ID: rs6323) in the recruited cohort of 482 subjects. Conclusion: The selected VNTR and SNP appeared to be significantly associated with aggression These VNTRs and SNP have not been studied previously in the Pakistani population, they represent a unique ethnic group. Association of variable number of tandem repeats (VNTR) and T941G polymorphism of monoamine oxidase (MAO-A) gene with aggression in Pakistani subjects.

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