Abstract
BackgroundTribbles 3 (TRB3) affects insulin signalling by inhibiting insulin-stimulated Akt phosphorylation and subsequent activation. A single nucleotide polymorphism located in the second extron of the human TRB3 gene is thought to be associated with insulin resistance. The latter is a core abnormality in PCOS independent of obesity. The present study was designed to clarify the relationships of TRB3 Q84R polymorphism with PCOS in a Chinese women group.MethodsA case-control study with two groups: PCOS group (n = 336) and control group of infertility women for tubal and/or male factor (n = 116) was performed. Genotyping of the TRB3 R84 variant was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).ResultsThe frequency of genotype QQ in PCOS women was significantly lower, while genotype QR and RR were significantly higher than that in control group (p < 0.05). However, the difference disappeared after adjustment for BMI. At glucose1h, glucose2h and insulin2h point, the difference between QQ individuals and R84 allele carriers in PCOS women reached statistical significance during OGTT (p < 0.05).ConclusionsTRB3 Q84R polymorphism is associated with obesity and especially glucose metabolism and not associated with polycystic ovary syndrome because of compositional characteristics of phenotype in Chinese PCOS women.
Highlights
Tribbles 3 (TRB3) affects insulin signalling by inhibiting insulin-stimulated Akt phosphorylation and subsequent activation
The current study aims at examining the relationships of TRB3 Q84R polymorphism with insulin resistance in a group of Chinese women patients with Polycystic ovary syndrome (PCOS)
Mann-Whitney U-test; Data are described in median; BMI:body mass index; Luteinizing hormone (LH):luteinizing hormone; FSH: follicle stimulating hormone; T:total serum testosterone;Glu0:fasting glucose; CHO:total cholesterol; TG:triglycerides; HDL: HDL-cholesterol; LDL: LDL-cholesterol. *: p < 0.05
Summary
Tribbles 3 (TRB3) affects insulin signalling by inhibiting insulin-stimulated Akt phosphorylation and subsequent activation. A single nucleotide polymorphism located in the second extron of the human TRB3 gene is thought to be associated with insulin resistance. The latter is a core abnormality in PCOS independent of obesity. Polycystic ovary syndrome (PCOS) is a common endocrine disorder with amenorrhoea (or oligomenorrhoea), hyperandrogenism, hirsutism, obesity and insulin resistance (IR). It affects 5-10% reproductive aged women and is a leading cause of female infertility [1]. It is well established that hyperinsulinemia and insulin resistance are common biochemical features of PCOS independent of obesity [5]
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