Abstract
Background and objective: Takayasu's arteritis is a disease that has been associated with tuberculosis, based upon the presence of Langerhans giant cells and granulomas. ATP treatment of mycobacteria infected macrophages induces apoptosis mediated via the P2X7 pathway. Here we evaluated if the polymorphisms of the P2X7 receptors are associated to the presence of tuberculosis in Mexican patients with Takayasu´s arteritis. Methods: 63 aortic samples, 33 from Takayasu´s and 30 from tuberculosis subjects were studied. Histological analysis was made and the sequence of the gene IS6110 which identifies the presence of Mycobacterium tuberculosis was amplified. Polymorphisms of the A1513C and -762 C/T receptors for the P2X7 gene were analyzed. Results: The analysis showed a high percentage of extra pulmonary lesions (36.3%) in the Takayasu´s group. No significant differences between the studied genotypes were found in any of the models analyzed or in any of the two P2X7 polymorphisms analyzed. Moreover, an increase in the 1513C allele in presence of IS6110 gene in the Takayasu´s group was observed when comparing with the group of tuberculosis. Conclusion: We found a high percentage of extra pulmonary tuberculosis in subjects with Takayasu´s arteritis; this increase was associated with a high frequency in the allele 1513C in the presence of IS6110 gene. Thus, these alleles may confer a significant susceptibility at the aortic level in the Mexican population having these diseases.
Highlights
Background and objectiveTakayasu's arteritis is a disease that has been associated with tuberculosis, based upon the presence of Langerhans giant cells and granulomas
We demonstrated that patients with Takayasu’s arteritis and tuberculosis lesions in the aortic tissues had the IS6110 gene, demonstrating that in both diseases the mycobacterium was present [11]
Here we studied the association of P2X7 receptor polymorphisms (A1513C and -762 C/T) and the presence of IS6110 gene as a proof of the presence tuberculosis in Mexican patients with Takayasu’s arteritis
Summary
63 aortic samples, 33 from Takayasus and 30 from tuberculosis subjects were studied. Histological analysis was made and the sequence of the gene IS6110 which identifies the presence of Mycobacterium tuberculosis was amplified. A total of 63 aortic samples embedded in paraffin were chosen from 57,560 autopsy files that were reviewed in the pathology department of the National Institute of CardiologyIgnacio Chávez. Cases were excluded if: a) patients did not fill the clinical diagnostic criteria, or b) if it was not possible to obtain the right aortic tissue or c) if DNA could not be suitably extracted or d) if expedients were incomplete. The second group consisted of thirty samples from patients with tuberculosis (TB), diagnosed by positive cultures of secretions or tissue samples and confirmed by bacilloscopy. The positive control for the analysis was a sample of M. tuberculosis obtained from cell lines extracted from strains of M. tuberculosis HRv37, provided by the Department of Cellular Biology of the National Institute of Cardiology (Figure 1)
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