Abstract
BackgroundEndothelial lipase (EL) is a major determinant of high-density lipoprotein-cholesterol (HDL-C) metabolism, but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations. Bai Ku Yao is an isolated subgroup of the Yao minority in China. This study was designed to detect the association of LIPG 584C > T (rs2000813) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.MethodsA total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples. Genotyping of the LIPG 584C > T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing.ResultsThe levels of serum total cholesterol (TC), HDL-C, low-density lipoprotein cholesterol (LDL-C) and apolipoprotein (Apo) AI and ApoB were lower in Bai Ku Yao than in Han (P < 0.05 - 0.001). The frequency of C and T alleles was 73.5% and 26.5% in Bai Ku Yao, and 67.9% and 32.1% in Han (P < 0.01); respectively. The frequency of CC, CT and TT genotypes was 50.4%, 46.2% and 3.4% in Bai Ku Yao, and 41.4%, 53.1% and 5.5% in Han (P < 0.01); respectively. Serum HDL-C levels in both ethnic groups were different among the three genotypes (P < 0.05 for each). Serum TC levels in both ethnic groups were also different between the CC and CT/TT genotypes (P < 0.05 for each). The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers. Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotypes in Bai Ku Yao (P < 0.05 for each), whereas the levels of TC and HDL-C were associated with genotypes in Han Chinese (P < 0.05 and P < 0.01). Serum lipid parameters were also correlated with several environmental factors in the both ethnic groups.ConclusionsThe frequency of LIPG 584T allele is lower in Bai Ku Yao than in Han Chinese. The LIPG 584T allele is associated with increased serum HDL-C, TC and ApoB levels. The differences in serum HDL-C, TC and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of LIPG 584C > T or different LIPG-enviromental interactions.
Highlights
Many epidemiological and clinical studies have shown that dyslipidemia, including high levels of plasma or serum total cholesterol (TC) [1,2], triglyceride (TG) [3,4], low-density lipoprotein cholesterol (LDL-C) [5,6] and apolipoprotein B (ApoB) [7,8], and low levels of high-density lipoprotein cholesterol (HDL-C) and ApoAI [9,10], is strongly associated with an increased risk of coronary artery disease (CAD)
The levels of body height, weight, BMI, systolic blood pressure, diastolic blood pressure, pulse pressure, serum TC, high-density lipoprotein-cholesterol (HDL-C), LDL-C, ApoAI, and ApoB were lower in Bai Ku Yao than in Han (P < 0.05 - 0.001), whereas the percentage of subjects who consumed alcohol was higher in Bai Ku Yao than in Han (P < 0.05)
There was no significant difference in the levels of LDL-C, ApoB, and the ratio of ApoAI to ApoB among the three genotypes in the both ethnic groups (P > 0.05 for all)
Summary
Many epidemiological and clinical studies have shown that dyslipidemia, including high levels of plasma or serum total cholesterol (TC) [1,2], triglyceride (TG) [3,4], low-density lipoprotein cholesterol (LDL-C) [5,6] and apolipoprotein B (ApoB) [7,8], and low levels of high-density lipoprotein cholesterol (HDL-C) and ApoAI [9,10], is strongly associated with an increased risk of coronary artery disease (CAD). Endothelial lipase (protein: EL; gene: LIPG) is a member of the triglyceride lipase family of proteins that includes lipoprotein lipase and hepatic lipase, and it exhibits a conserved catalytic triad, heparin-binding properties, lipid-binding domains, and cysteine residues [21]. Studies in mouse models showed that a decrease in EL expression and activity, by gene deletion of LIPG in knockout mice [26,27] and by antibody inhibition [28], resulted in significant increases in plasma HDL-C in mice. Endothelial lipase (EL) is a major determinant of high-density lipoprotein-cholesterol (HDL-C) metabolism, but the association of endothelial lipase gene (LIPG) polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations. This study was designed to detect the association of LIPG 584C > T (rs2000813) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
