Association of the APOA1 rs964184 SNP and serum lipid traits in the Chinese Maonan and Han populations

Ling Qiu,Fen-Han Zhang,Eksavang Khounphinith,Rui-Xing Yin,De-Zhai Yang,Shang-Ling Pan

doi:10.1186/s12944-018-0759-8

Abstract

BackgroundMaonan nationality belongs to a mountain ethnic minority in China. Little is known about the association of apolipoprotein A1 gene (APOA1) rs964184 single nucleotide polymorphism (SNP) and serum lipid levels in this population. The aim of this study was to detect the association of the APOA1 rs964184 SNP and several environmental factors with serum lipid profiles in the Chinese Maonan and Han populations.MethodsGenotypes of the APOA1 rs964184 SNP in 867 individuals of Maonan nationality and 820 participants of Han nationality were determined by polymerase chain reaction and restriction fragment length polymorphism, combined with gel electrophoresis, and confirmed by direct sequencing.ResultsThe frequencies of CC, CG and GG genotypes of the APOA1 rs964184 SNP were 68.86, 29.18 and 1.96% in the Maonan population, and 63.78, 30.85 and 5.37% in the Han population (P < 0.001). The frequency of the G allele was 16.55% in Maonan and 20.79% in Han (P < 0.001). The G allele carriers had lower high-density lipoprotein cholesterol (HDL-C) levels in Maonan and higher triglyceride (TG) levels in Han peoples than the G allele non-carriers. Subgroup analyses showed that the G allele carriers had lower HDL-C levels in both Maonan males and females; and lower apolipoprotein (Apo) A1 levels and the ApoA1/ApoB ratio in Han males than the G allele non-carriers. Serum lipid parameters in the two ethnic groups were also associated with several environmental factors.ConclusionsThe present study reveals that there may be a racial/ethnic- and/or gender-specific association between the APOA1 rs964184 SNP and serum lipid parameters in our study populations.Trial registrationRetrospectively registered.

Highlights

Maonan nationality belongs to a mountain ethnic minority in China
Results of electrophoresis and genotyping After the genomic deoxyribonucleic acid (DNA) of the samples was amplified using Polymerase chain reaction (PCR) and visualized with 2% agarose gel electrophoresis, the products of 320 bp nucleotide sequences were observed in all samples (Fig. 1)
Multivariable linear regression analyses with stepwise modeling were used to determine the correlation between the genotypes (CC = 1, CG = 2 and GG = 3) and several environmental factors with serum lipid levels in males and females of Maonan and Han populations

Summary

Introduction

Little is known about the association of apolipoprotein A1 gene (APOA1) rs964184 single nucleotide polymorphism (SNP) and serum lipid levels in this population. The aim of this study was to detect the association of the APOA1 rs964184 SNP and several environmental factors with serum lipid profiles in the Chinese Maonan and Han populations. Nowadays cardiovascular disease (CVD) becomes the primary cause of disability among adults found in some studies, and coronary artery disease (CAD) continues to be the leading cause of death and years of life lost around the world [1, 2]. It is well established that abnormal serum lipid levels are concerned with genetic and multiple environmental factors and their interactions [11]. There is another study showed that the pathway of HDL-C metabolism involves Apo, and ApoA1 is the most abundant component

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