Association of TGFb1 Haplotypes with Quantitative Traits in Allergic Reactions

Abstract

RationaleWe have previously reported that a TGFb1 haplotype in promoter region was associated with higher risk of allergy in Caucasians and lower risk of allergy in Asians. The associations of the TGFb1 haplotypes with allergy related quantitative traits were further explored to understand the mechanism.MethodsThe plasma TGFb1, total IgE, CD23 and cell proliferation in response to antigens were measured in allergic patients and healthy subjects. Multiple regression analyses were conducted to test the association between the haplotypes and these quantitative traits.ResultsThe haplotype TTA (rs224175, rs1800469 and rs1982072) was found to be significantly associated with increased plasma TGFb1 concentration accounting for about 7.4% of variance in plasma TGFb1 while allergy and race were considered as covariates (p = 0.0277 < 0.05). There is no significant difference in terms of TTA related increase in TGFb1 between Asians and Caucasians. However, the Asians seem to have lower plasma TGFb1 than Caucacians (difference: 267 (±148.37)pg/ml, p = 0.0762). The haplotype TTA was not associated with plasma total IgE and CD23, but it was associated with lower basic level of cell proliferation in PBMC, maximum cell proliferation to tetanus, cat and grass allergens.ConclusionsTGFb1 concentration associated with haplotype TTA may be crucial in mediating allergy development. The different TGFb1 concentration may account for the difference in allergy risk between Asians and Caucasians. RationaleWe have previously reported that a TGFb1 haplotype in promoter region was associated with higher risk of allergy in Caucasians and lower risk of allergy in Asians. The associations of the TGFb1 haplotypes with allergy related quantitative traits were further explored to understand the mechanism. We have previously reported that a TGFb1 haplotype in promoter region was associated with higher risk of allergy in Caucasians and lower risk of allergy in Asians. The associations of the TGFb1 haplotypes with allergy related quantitative traits were further explored to understand the mechanism. MethodsThe plasma TGFb1, total IgE, CD23 and cell proliferation in response to antigens were measured in allergic patients and healthy subjects. Multiple regression analyses were conducted to test the association between the haplotypes and these quantitative traits. The plasma TGFb1, total IgE, CD23 and cell proliferation in response to antigens were measured in allergic patients and healthy subjects. Multiple regression analyses were conducted to test the association between the haplotypes and these quantitative traits. ResultsThe haplotype TTA (rs224175, rs1800469 and rs1982072) was found to be significantly associated with increased plasma TGFb1 concentration accounting for about 7.4% of variance in plasma TGFb1 while allergy and race were considered as covariates (p = 0.0277 < 0.05). There is no significant difference in terms of TTA related increase in TGFb1 between Asians and Caucasians. However, the Asians seem to have lower plasma TGFb1 than Caucacians (difference: 267 (±148.37)pg/ml, p = 0.0762). The haplotype TTA was not associated with plasma total IgE and CD23, but it was associated with lower basic level of cell proliferation in PBMC, maximum cell proliferation to tetanus, cat and grass allergens. The haplotype TTA (rs224175, rs1800469 and rs1982072) was found to be significantly associated with increased plasma TGFb1 concentration accounting for about 7.4% of variance in plasma TGFb1 while allergy and race were considered as covariates (p = 0.0277 < 0.05). There is no significant difference in terms of TTA related increase in TGFb1 between Asians and Caucasians. However, the Asians seem to have lower plasma TGFb1 than Caucacians (difference: 267 (±148.37)pg/ml, p = 0.0762). The haplotype TTA was not associated with plasma total IgE and CD23, but it was associated with lower basic level of cell proliferation in PBMC, maximum cell proliferation to tetanus, cat and grass allergens. ConclusionsTGFb1 concentration associated with haplotype TTA may be crucial in mediating allergy development. The different TGFb1 concentration may account for the difference in allergy risk between Asians and Caucasians. TGFb1 concentration associated with haplotype TTA may be crucial in mediating allergy development. The different TGFb1 concentration may account for the difference in allergy risk between Asians and Caucasians.