Association of STAT6 and ADAM33 single nucleotide polymorphisms with asthma bronchiale and IgE level and its possible epigenetic background

Marek Godava,Marie Markova,Radek Vrtel,Jana Bohmova,Frantisek Kopriva,Michaela Cvanova,Jan Muzik,Eva Schneiderova,Radek Vodicka,Ladislav Dusek

doi:10.5507/bp.2012.009

Abstract

ADAM33 and STAT6 belong to the candidate genes that have been commonly associated with asthma, bronchial hyperresponsiveness or IgE levels. Our objective was to assess the association of 11 SNPs of the ADAM33 and 6 of the STAT6 and their haplotypes with IgE levels and asthma. We also evaluated the possible role of parental origin of haplotypes on IgE levels. We enrolled 109 children with asthma and 45 healthy controls. Genotyping was performed by TaqMan probes and confirmed by sequencing. Haplotype construction was based on the knowledge of parental genotypes and also inferred by using the EM algorithm and Bayes' theorem. None of the SNPs were associated with elevated IgE level or asthma. We found that the most frequent STAT6 haplotype ATTCAA (built from rs324012, rs324011, rs841718, rs3024974, rs3024974, rs4559 SNPs, respectively) was associated with elevated total IgE levels (P=0.01) and this haplotype was predominantly transmitted paternally (P<0.001). We compared our results with those of studies performed on German and Australian Caucasian populations and found that rs324011, rs3024974 and rs4559 SNPs in STAT6 should have a major effect on IgE levels. Therefore, we suggest the TCA haplotype alone (built from rs324011, rs3024974 and rs4559 SNPs, respectively) in STAT6 is associated with total IgE elevation. The influence of paternal origin of the STAT6 haplotype on IgE levels is surprising but the exact role of possible paternal imprinting in STAT6 regulation should be investigated and confirmed in future studies.

Highlights

Asthma is defined as a chronic inflammatory airway disease with recurrent bronchoconstriction caused by stimuli that would otherwise not cause airway narrowing in most individuals
Genotypes and allele frequencies of the studied genes ADAM33 and signal transducer and activator of transcription 6 (STAT6) SNPs were successfully determined in all studied patients, their relatives and in the control group (45 children) as well
For each of the studied genes we found three of the most common haplotypes; they were marked as a1-3 for ADAM33 and s1-3 for STAT6, in descending order according to their frequency rate

Summary

Introduction

Asthma is defined as a chronic inflammatory airway disease with recurrent bronchoconstriction caused by stimuli that would otherwise not cause airway narrowing in most individuals. AHR may represent more than a single entity It may reflect a common physiological pathway of several mechanisms leading to a lowered threshold of airway narrowing to bronchoconstrictor stimuli. Our objective was to assess the association of 11 SNPs of the ADAM33 and 6 of the STAT6 and their haplotypes with IgE levels and asthma. We found that the most frequent STAT6 haplotype ATTCAA (built from rs324012, rs324011, rs841718, rs3024974, rs3024974, rs4559 SNPs, respectively) was associated with elevated total IgE levels (P=0.01) and this haplotype was predominantly transmitted paternally (P

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