Association of SLC34A1 and RGS14 polymorphisms with serum phosphorus levels in chronic hepatitis B patients following long-term treatment of adefovir dipivoxil

Abstract

Objective To investigate the association of SLC34A1 (rs6420094) and RGS14(rs4074995) polymorphisms with serum level of phosphorus in chronic hepatitis B virus (HBV) infection patients following long-term adefovir dipivoxil (ADV) treatment. Methods Ninety one patients with chronic HBV infections admitted in Third Hospital of Hebei Medical University during October 2012 and August 2013 were enrolled in the study. All patients received ADV treatment (monotherapy or combination therapy) for at least two years, among whom 31 had hypophosphatemia and 60 had normal serum phosphorus levels. rs6420094 and rs4074995 were genotyped by polymerase chain reaction-restrictive fragment length polymorphism (PCR-RFLP) analysis. The relationship between allele frequency and serum phosphorus concentration was analyzed by Chi-Square test. Results In hypophosphatemia group, there were 13, 13, and 5 patients with genotype A/A, A/G and G/G at rs6420094, respectively; while in patients with normal serum phosphorus levels, there were 35, 24 and 1 patients with genotype A/A, A/G and G/G at rs6420094. The frequency of allele A at rs6420094 in patients with normal serum level of phosphorus was higher than that in hypophosphatemia group (78.3% vs. 62.9%, χ2=4.947, P 0.05). Conclusion The polymorphism of rs6420094 gene may be associated with the serum level of phosphorus in patients with chronic HBV infections following long-term treatment of ADV. Key words: Hepatitis B, chronic; Polymorphism, single nucleotide; Phosphorus; Adefovir dipivoxil