Association of Single-Nucleotide Polymorphism (rs833061) of Vascular Endothelial Growth Factor with Breast Cancer Risk

Abstract

Background: Breast cancer is the most common cancer among women and one of the most important causes of death among them. Angiogenesis is an important step for primary tumor growth, invasiveness, and metastases. Vascular endothelial growth factor (VEGF) is involved in angiogenesis associated with tumors. Objective: To investigate the association of single nucleotide polymorphisms (SNPs) of VEGF gene located in the promoter region at position 460T/C (rs833061) with the risk of breast cancer. Patients and Methods: This study was conducted on (40) Egyptian female patients who were diagnosed as breast cancer according to histopathological examination of breast biopsy (Group 1, Patients), in addition to (40) age-matched apparently healthy females serving as controls (Group II, Controls). Detection of SNP of VEGF gene located in the promoter region 460T/C (rs833061) was done by real-time polymerase chain reaction technique. Results: There was no significant difference in frequency of the (C/C, T/C and T/T) genotypes of VEGF 460T/C (rs833061) in group I compared with group II (p>0.05). None of the studied VEGF genotypes had shown significant difference in distribution related to clinico-pathological parameters as tumor size, histological grade, hormone receptors, HER2/new status, regional lymph node and distant metastasis (p>0.05). Conclusion: This study revealed that there was no significant association of the (C/C, T/C and T/T) genotypes of VEGF 460T/C (rs833061) and breast cancer risk. Moreover, VEGF polymorphism 460T/C (rs833061) was not associated with breast cancer patients’ clinico-pathological characteristics and tumor markers levels.