Abstract
BackgroundThe roles of FTO gene and the level of serum 25-OH-vitamin D in obesity are frequently reported. This study aimed to investigate the interactions of serum 25-OH-vitamin D level, FTO and IRX3 genes expression, and FTO genotype in obese and overweight boys.MethodsThis study was carried out on the 120 male adolescents with overweight in Tehran, Iran. Blood samples were collected from the participants in order to evaluate the serum level of 25-OH-vitamin D, the expression level of FTO and IRX3 genes, and FTO genotype for rs9930506 at baseline and after 18 weeks of the study.ResultsIn general, no significant association was found between serum 25-OH-vitamin D level and IRX3 and FTO genes expression. The results of linear regression on the relationship between 25-OH-vitamin D serum level and FTO and IRX3 genes expression based on FTO genotypes for rs9930506 indicated that in AA/AG genotype carriers, serum 25-OH-vitamin D level was positively associated with FTO gene expression (B = 0.07, p = 0.02) and inversely associated with IRX3 gene expression (B = − 0.07, p = 0.03). In GG carriers, serum 25-OH-vitamin D level was not associated with expression of IRX3 and FTO genes.ConclusionThere are significant interactions between 25-OH-vitamin D and the expression of FTO and IRX3 genes in the subset of obese patients with specific genotypes for FTO rs9930506. There was no association between serum 25-OH-vitamin D levels and the expression of FTO and IRX genes in individuals with a homozygous genotype for the risk allele of the FTO gene polymorphism.
Highlights
Over 430 genes have been recognized to be associated with obesity and overweight [1, 2]
Five ml of Blood samples were collected from the participants in order to evaluate the serum level of 25-OH-vitamin D, the expression levels of FTO and Iroquoisrelated Homeobox 3 (IRX3) genes, and FTO genotype at the beginning and after 18 weeks of the study
Linear regression identified no significant association between serum 25-OH-vitamin D level and FTO and IRX3 genes expression (β = 0.03, P = 0.25 and β = − 0.16, P = 0.34, respectively) (Table 3)
Summary
Over 430 genes have been recognized to be associated with obesity and overweight [1, 2]. FTO (Fat mass and obesity-associated) gene with a high rate of genetic variations may has a key role in life-long obesity [3]. The FTO is the best-known obesity-related gene which can exert its effects through several mechanisms such as impacts on eating behaviors [4], energy. Age-related effects of FTO gene on BMI have been reported in studies conducted on children, which was characterized by disturbed adiposity rebound and higher BMI in adulthood [6]. The roles of FTO gene and the level of serum 25-OH-vitamin D in obesity are frequently reported. This study aimed to investigate the interactions of serum 25-OH-vitamin D level, FTO and IRX3 genes expression, and FTO genotype in obese and overweight boys
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