Abstract
The TaqI B (rs708272) single-nucleotide variant, i.e., the +279 G/A substitution in intron 1 of the CETP gene, is actively investigated as a risk factor of lipid metabolism disorders. The aim of this study was to analyze the association of rs708272 with lipid parameters and the risk of myocardial infarction in the population of Western Siberia (Russia). The study population was selected from a sample surveyed within the framework of the Health, Alcohol and Psychosocial Factors In Eastern Europe (HAPIEE) study (9360 participants, >90% white, aged 45–69 years, males: 50%). In total, 3132 randomly selected patients were included. Plasma lipid levels were determined by standard enzymatic assays. Rs708272 was analyzed by RT-PCR via TaqMan single-nucleotide polymorphism (SNP) Genotyping Assays (Thermo Fisher Scientific, USA). The frequencies of rs708272 genotypes AA (homozygote), AG (heterozygote), and GG were 0.21, 0.49, and 0.30, respectively, in this population. Allele A frequency was 0.46. We found an association of allele G with low levels of high-density lipoprotein cholesterol and a high index of atherogenicity in this population (p < 0.001 and p < 0.001, respectively). Allele G was significantly associated with the risk of myocardial infarction among the male participants (odds ratio 1.96, 95% confidence interval 1.208–3.178, p = 0.008) and in the study population (odds ratio 1.465, 95% confidence interval 1.028–2.087, p = 0.036). Thus, rs708272 is associated with myocardial infarction in the white population of Western Siberia (Russia).
Highlights
Cardiovascular diseases (CVDs) are the leading cause of death in developed countries [1]
We present the results of an examination of a white population regarding a possible association of rs708272 with clinical and biochemical parameters
We found that some changes in genomic DNA sequence are independent risk factors of myocardial infarction, in agreement with the findings of other studies [17,18,19,20,21,22,23,24]
Summary
Cardiovascular diseases (CVDs) are the leading cause of death in developed countries [1]. There is a correlation between the level of high-density lipoprotein cholesterol (HDL-C) and the risk of CVD; this correlation is in part explainable by the participation of HDL in reverse cholesterol transport [3]. The latter is a process of transfer of cholesterol esters via HDL particles into the liver, with subsequent cholesterol oxidation to bile acids. One of the key proteins taking part in this phenomenon is cholesterol ester transport protein (CETP) This is a hydrophobic glycoprotein regulating the reverse cholesterol transport from peripheral tissues to the liver and participating in the Biomolecules 2019, 9, 739; doi:10.3390/biom9110739 www.mdpi.com/journal/biomolecules
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