Association of rs1800629 tumor necrosis factor alpha polymorphism with risk of prostate tumors of Iraqi patients

Abstract

Several factors associated with prostate cancer, one of them is inflammation. Tumor necrosis factor alpha (TNF-α) cytokine has an important role in inflammation. The aim of this study was to investigate the effect of TNF-α-308 (G/A) polymorphism on the potential danger of prostate tumors on Iraqi prostate tumors patients. In this research, the gene polymorphism of TNF-α in sixty patients, thirty of which were diagnosed with prostate cancer(PCa) and thirty with benign prostate hyperplasia (BPH), and also in thirty healthy volunteers serving as the control group was studied. Specific primers were used for the amplification of the promoter region of TNF-α (rs1800629) by Polymerase chain reaction (PCR). Restriction fragment length polymorphism (RFLP) was used for the determination of the alleles frequencies associated with each group of the study subjects. The result of RFLP showed that GA allele was correlated with both BPH and PCa, leading to a conclusion that GA allele in TNF-α gene polymorphism may be considered a risk factor for developing PCa.