Abstract
ObjectiveTo investigate whether the changes in collagen gene expression in osteoarthritic (OA) human chondrocytes are associated with changes in the DNA methylation status in the COL2A1 enhancer and COL9A1 promoter.MethodsExpression levels were determined using quantitative reverse transcription–polymerase chain reaction, and the percentage of DNA methylation was quantified by pyrosequencing. The effect of CpG methylation on COL9A1 promoter activity was determined using a CpG-free vector; cotransfections with expression vectors encoding SOX9, hypoxia-inducible factor 1α (HIF-1α), and HIF-2α were carried out to analyze COL9A1 promoter activities in response to changes in the methylation status. Chromatin immunoprecipitation assays were carried out to validate SOX9 binding to the COL9A1 promoter and the influence of DNA methylation.ResultsAlthough COL2A1 messenger RNA (mRNA) levels in OA chondrocytes were 19-fold higher than those in the controls, all of the CpG sites in the COL2A1 enhancer were totally demethylated in both samples. The levels of COL9A1 mRNA in OA chondrocytes were 6,000-fold lower than those in controls; 6 CpG sites of the COL9A1 promoter were significantly hypermethylated in OA patients as compared with controls. Treatment with 5-azadeoxycitidine enhanced COL9A1 gene expression and prevented culture-induced hypermethylation. In vitro methylation decreased COL9A1 promoter activity. Mutations in the 5 CpG sites proximal to the transcription start site decreased COL9A1 promoter activity. Cotransfection with SOX9 enhanced COL9A1 promoter activity; CpG methylation attenuated SOX9 binding to the COL9A1 promoter.ConclusionThis first demonstration that hypermethylation is associated with down-regulation of COL9A1 expression in OA cartilage highlights the pivotal role of epigenetics in OA, involving not only hypomethylation, but also hypermethylation, with important therapeutic implications for OA treatment.
Highlights
Supported by the NIH, Wessex Medical Research, and the Biotechnology and Biological Sciences Research Council
COL2A1 messenger RNA levels in OA chondrocytes were 19-fold higher than those in the controls, all of the CpG sites in the COL2A1 enhancer were totally demethylated in both samples
This first demonstration that hypermethylation is associated with down-regulation of COL9A1 expression in OA cartilage highlights the pivotal role of epigenetics in OA, involving hypomethylation, and hypermethylation, with important therapeutic implications for OA treatment
Summary
Expression levels were determined using quantitative reverse transcription–polymerase chain reaction, and the percentage of DNA methylation was quantified by pyrosequencing. Human chondrocytes were isolated from the articular cartilage of femoral heads obtained at the time of surgery for total hip replacement (12 OA patients [4 men and 8 women], with a mean Ϯ SD age of 72.4 Ϯ 7.9 years) or for femoral neck fracture (10 control subjects [2 men and 8 women], with a mean Ϯ SD age of 79.2 Ϯ 5.8 years), as previously described [27]. Chondrocytes were cultured for 48 hours at 37°C at a density of 2–4 ϫ 105 cells in Dulbecco’s modified Eagle’s medium (DMEM)/ F-12 medium supplemented with 5% fetal calf serum, 1% insulin–transferrin–selenium, 100 units/ml of penicillin, 100 g/ml of streptomycin, and 100 g/ml of ascorbic acid in an atmosphere of 5% CO2
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