Abstract
To investigate the correlation of Adenine-1012-Guanine (rs4516035) promoter region polymorphism of vitamin-D receptor gene with serum levels of omentin-1, vitamin-D and vitamin-D receptor protein in patients with coronary artery disease. The case-control study was conducted from January to June 2020 at the cardiac unit of Civil hospital Karachi (CHK), and comprised coronary artery disease patients and controls. The tetra-primer amplification refractory mutation system polymerase chain reaction method was used to genotype Adenine-1210Guanine polymorphism in the vitamin D receptor gene. Serum levels of omentin-1, vitamin-D, and vitamin-D receptor protein were measured in both the groups using an enzyme-linked immunosorbent assay. Data was analysed using SPSS 17. Of the 1,000 subjects, there were 500(50%) cases; males 306(61.2%) and 194(38.8%) females with overall mean age of 51.08±9.55 years. The remaining 500(50%) were controls; 290(58%) males and 210(42%) females with overall mean age of 50.9±10.78 years. The mutant Guanine allele was more prevalent in controls 261(52.2%), and had a non-significant correlation with coronary artery disease (p=0.45). Among the cases, the wild Adenine-Adenine genotype had a higher prevalence 402(80.4%) and had a significant correlation with coronary artery disease (p<0.001). The heterozygous genotype Adenine-Guanine was significantly more predominant among the controls 346(69.2%) compared to the cases 66(13.2) (p=0.002). Adenine-1012-Guanine polymorphism in the vitamin-D receptor gene was found to be a protective polymorphism for coronary artery disease in the recessive model.
Highlights
Coronary artery disease (CAD) is the most prevalent cardiovascular disorder, characterised by thrombotic occlusion, or atherosclerotic plaque, of at least one of the major coronary artery
Adenine-1012-Guanine polymorphism in the vitamin-D receptor gene was found to be a protective polymorphism for coronary artery disease in the recessive model
While sustained efforts have been made to determine the protective role of vitamin D in cardiovascular disorders, the molecular dimensions of this relationship remain unrevealed
Summary
Coronary artery disease (CAD) is the most prevalent cardiovascular disorder, characterised by thrombotic occlusion, or atherosclerotic plaque, of at least one of the major coronary artery. Various hereditary and biological elements are associated with the fihydroxycholecalciferol) is a fat-soluble vitamin obtained from sun exposure, food and supplements[2]. Literature has shown that deficient vitamin D levels affect the musculoskeletal, immune and cardiovascular systems[3,4]. While sustained efforts have been made to determine the protective role of vitamin D in cardiovascular disorders, the molecular dimensions of this relationship remain unrevealed. Vitamin D executes its biological activities by binding to the vitamin D receptor (VDR), a transcription factor found in most body cells. VDR is coded by extremely polymorphic vitamin
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