Abstract
Sera from patients with rheumatoid arthritis (RA) were tested for cold reactive lymphocytotoxic antilymphocyte antibodies (ALA). Of 25 sera, 3 had reactivity with resting lymphocytes, 7 of 25 sera reacted with cultured and PWM-activated lymphocytes, and 9 of 25 sera had reactivity with Con A-activated lymphocytes. Positive sera had minimal increases in ALA titer with cultured cells and marked increases with mitogen-activated target cells. ALA against activated lymphocytes were found to be predominantly IgM cold reactive, complement-dependent antibodies. Increased ALA reactivity was directed against lymphoblasts which were purified on PVP-coated colloidal silica (Percoll) gradients. RA sera reactivity against lymphoblasts was also increased using an indirect immunofluorescent technique. ALA activity against activated lymphocytes was absorbed with fresh mononuclear cells.
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