Abstract
Objective To study the association between polymorphisms within angiotensin Ⅱ receptor genes (AT1 R,AT2R) and postoperative recovery of blood pressure (BP) in patients with aldosterone producing adenoma (APA).Methods Genomic DNA was extracted from adenoma tissues of 148 APA patients.Minor groove junction (MGB)-Taqman probe was used to detect genotype of 4 single nucleotide polymorphism(SNP) loci (rs5182,rs5186,rs5194 and rs1403543) within AT1R and AT2 R genes.The postoperative recovery of BP in APA patients was followed up.After adrenalectomy,patients were categorized as cured if they had no hypertension [defined as systolic blood pressure (SBP) ＜ 140 mm Hg (1 mm Hg =0.133 kPa) and diastolic blood pressure (DBP) ＜90 mm Hg] and were not given any antihypertensive medications 6 months after surgery,or they were categorized as having persistent hypertension.Hardy-Weinberg equilibrium (HWE) and genotype frequencies of each polymorphism locus were determined by SNPassoc in R statistics program 2.7.0 software package.A P value ＜0.05 or odds ratio (OR) 1.00 not in the range of 95％ confidence interval (CI) implicated statistically significant difference.Results Total of 128 APA patients were followed up,including 72 patients with idea control of postoperative BP and 56 with persistent hypertension postoperatively.The distribution of genotypes of each locus was in accordance with Hardy-Weinberg Equilibrium (HWE) in the above two groups (P ＞0.05).Of the 4 SNPs,only rs1403543 locus in AT2R gene was associated with persistently postoperative hypertension of APA patients.APA patients with homozygotic genotype GG had an increased risk of persistently postoperative hypertension compared to patients with AA or GA genotype (OR =5.00,95％ CI =1.31-19.15,P ＜0.05).Conclusion Polymorphisms at rs1403543 locus in AT2R gene may be a predictor for postoperative BP recovery of APA patients. Key words: Aldosterone-producing adenoma; Angiotensin Ⅱ receptor; Polymorphism; Blood pressure
Published Version
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