Abstract
The process of dietary fat absorption (DFA) by the absorptive cells of the intestine, enterocytes, is important in regulating energy balance in whole animals. Recently, we found that enterocytes dynamically store triacylglycerols (TGs) in cytoplasmic lipid droplets (CLDs) during DFA. To further our understanding of the process of DFA and regulation of TG rich CLD synthesis and catabolism within enterocytes, we investigated the expression and localization of PAT protein family members in enterocytes of mice under acute and chronic dietary challenges. We found that adipophilin and TIP47 are the only PAT proteins expressed in mouse intestinal mucosa. In mice acutely and chronically challenged by dietary fat, we imaged the cellular localization of TIP47 and adipophilin using immunofluorescence in combination with Coherent anti‐Stokes Raman scattering microscopy (CARS) to image TG in CLDs We found that TIP47 is expressed in the cytoplasm under all conditions but only associates with CLDs after an acute challenge. Adipophilin, on the otherhand is only expressed and associated with CLDs in enterocytes of chronic high‐fat fed mice. These results suggest distinct roles for TIP47 and adipophilin in DFA and ultimately regulation of whole animal energy balance.Grant Funding SourcePurdue Research Foundation
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