ASSOCIATION OF OBESITY WITH RS1421085 AND RS9939609 POLYMORPHISMS OF FTO GENE WITH T2DM IN EGYPTIAN FEMALES

Adel A.-H Abdel Rahman ,Rizk Ahmed El Baz ,Ahmed E.‐S Abdel Megied ,Alaa Wafa ,Abeer Saad El Zekred

doi:10.22159/ijpps.2018v10i9.22747

Adel A.-H Abdel Rahman , Rizk Ahmed El Baz + Show 3 more

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https://doi.org/10.22159/ijpps.2018v10i9.22747

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Abstract

Objective: Obesity has been described as a worldwide increasing health problem and risk factor of various disorders including type 2 diabetes mellitus (T2DM). So, our study aim to determine of common variants of fat mass and obesity associated gene polymorphisms rs1421085 and rs 9939609; confers risk of obesity and type 2 diabetic mellitus in Egyptian females.Methods: In this population rs1421085 and rs9939609 polymorphisms of fat mass and obesity (FTO) gene were genotyped in 105 obese patients and 100 healthy controls with ages 14-60 y were collected from Medicine Specialized Hospital, Mansoura University, Egypt during the period between Jul.-Oct. 2016, genotyping of SNPs was performed by restriction fragment length polymorphism (RFLP) assay, fasting blood glucose, homeostasis model assessment of insulin resistance (HOMA IR), body mass index (BMI), waist-to-hip ratio (WHR) lipid profile was determined.Results: There was the significantly higher frequency of the AA compared to controls p=0.0001) of genotypers9939609. Also, cases have shown a significantly higher frequency of the C allele, p<0.00001) of rs1421085 genotype polymorphisms increased the risks of obesity. On the other hand, there were no significant correlations between genotypes and obesity-related (anthropometric body composition) parameters. Only the fasting blood glucose was significantly higher in the TA p=0.004).Conclusion: The FTO rs9939609 and rs1421085 single nucleotide polymorphisms (SNPs) was associated with increased risk of obesity in type 2 diabetic populations on Egyptian females.

Highlights

fat mass and obesity (FTO) gene was assigned to chromosome 16 at position 12.2 [1]
The fact that high levels of FTO expression are found in the brain, in the accurate nucleus of the hypothalamus, which is known to play a major role in controlling energy homeostasis and eating behaviour, together with new insights from recent in vitro studies indicating a possible role in energy expenditure, provides support for a role of FTO in the explanation of obesity development [3, 4]
Weight, cholesterol, triglycerides, low density liprotien (LDL), insulin, Homa IR, waist, hip and body mass index (BMI) were significantly higher in cases compared to controls (p0.05)

Summary

Introduction

FTO gene was assigned to chromosome 16 at position 12.2 [1]. FTO has a total length of 410509 bp and contains nine exons and several nucleotide polymorphisms [2].The fact that high levels of FTO expression are found in the brain, in the accurate nucleus of the hypothalamus, which is known to play a major role in controlling energy homeostasis and eating behaviour, together with new insights from recent in vitro studies indicating a possible role in energy expenditure, provides support for a role of FTO in the explanation of obesity development [3, 4].Obesity is considered a medical condition involving an accumulation of excess body fat potentially posing a threat to health and life of an individual. The prevalence of obesity is increasing worldwide in both developing and developed countries. It has become a serious epidemic health problem, estimated to be the fifth leading cause of modality at global level [5,6,7,8,9]. Studies in Chinese Han [10], Oceanic [11], and African populations [12] failed to confirm rs9939609 variant as a major contributor to obesity and type 2 diabetic (T2D). Studies in Korean, Malay, Japanese, Canadians of South Asian origin, and Chinese populations have reported the association of this SNP with BMI and obesity [13,14,15,16,17,18,19]. The disease association of the FTO variant varies with ethnicity and populations

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