Abstract
HomePlant DiseaseVol. 99, No. 11Association of Nerine yellow stripe virus With Mosaic Disease of Crinum asiaticum Ornamental Plant in India PreviousNext DISEASE NOTES OPENOpen Access licenseAssociation of Nerine yellow stripe virus With Mosaic Disease of Crinum asiaticum Ornamental Plant in IndiaS. Kumar, R. Raj, C. Kaur, and S. K. RajS. KumarSearch for more papers by this author, R. RajSearch for more papers by this author, C. KaurSearch for more papers by this author, and S. K. RajSearch for more papers by this authorAffiliationsAuthors and Affiliations S. Kumar R. Raj C. Kaur S. K. Raj , Plant Molecular Virology Laboratory, CPMB Division, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, Uttar Pradesh, India. Published Online:26 Aug 2015https://doi.org/10.1094/PDIS-02-15-0211-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Crinum asiaticum L. (Amaryllidaceae), commonly known as spider lily, is widely grown as an ornamental plant in gardens of tropical and subtropical countries for its long, strap-like, green leaves and clustered white flowers. During a survey in December 2013, severe mosaic symptoms were observed on a number of C. asiaticum plants growing in gardens at Noida and New Delhi cities of India. The symptoms were similar to those described earlier from Brazil on a potyvirus-infected Crinum sp. (Barboza et al. 2006); therefore, potyvirus infection was suspected. Electron microscopy was performed with leaf dip preparations of six randomly collected symptomatic leaf samples which revealed the presence of flexuous, rod-shaped, ∼700 × 12-nm virus particles, suggesting association of a potyvirus. These samples were further subjected to RT-PCR using potyvirus degenerate primers (Gibbs et al. 2003). RT-PCR resulted in the expected size (∼1.5 kb) amplicon in all symptomatic samples. The amplicons were cloned and sequenced, and sequence data were deposited as GenBank Accession Nos. KJ886933 (Nod-1), KM066970 (Nod-2), KM066968 (Nod-3), KJ886934 (Del-1), KM066971 (Del-2), and KM066969 (Del-3) isolates. Local alignments of the sequences of these isolates in NCBI revealed the presence of partial nuclear inclusion body (NIb), complete coat protein (CP) genes, and the 3′ UTR untranslated region (UTR) of a potyvirus. The isolates under study shared 98 to 99% sequence identity together and showed 78 to 99% identities with Nerine yellow stripe virus (NeYSV) isolates (FJ618537, DQ407932, EF362621, EF362622, EU042758, and JX865782) reported from New Zealand, The Netherlands, and USA. During phylogenetic analysis by MEGA (V.5.1) (Tamura et al. 2013), the isolates under study clustered together and showed close relationships with NeYSV isolates, and the potyvirus associated with mosaic disease of C. asiaticum was identified as NeYSV. The six and other nine symptomatic leaf samples of C. asiaticum were also tested by nucleic acid hybridization assay using the probe prepared from a clone of NeYSV (KJ886933), which gave strong signals of hybridization confirming presence of NeYSV in them. The probe may be used for the indexing of leaf samples collected from various locations and to understand the geographical distribution of NeYSV. A literature survey revealed the only report of association of an unidentified potyvirus with the mosaic disease of Crinum sp. from Brazil (Barboza et al. 2006) based on RT-PCR amplification of 2.0 kb product by potyvirus genus-specific primers. To best of our knowledge, this is the first report of natural occurrence of NeYSV on C. asiaticum in India. Because C. asiaticum is being propagated through bulbs, the dissemination of virus from nursery to nursery and from place to place is a major concern and now warrants the indexing of mother culture of C. asiaticum for NeYSV.
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