Association of NAD(P)H oxidase C242T gene polymorphism with lipid level and lipid metabolism of patients with cerebral infarction from Shongjiang Shanghai

Abstract

Objective To explore the relations of nicotinamide adenine dinucleotide phosphate(NAD[P]H)oxidase p22phox C242T gene polymorphism with hyperlipidemia and cerebral infarction. Methods A case-control association study containing 207 hyperlipidemia patients(collected from Physical Examination Center from March 2014 to March 2015), 185 cerebral infarction patients(collected from Department of Neurology from March 2014 to March 2015)and 183 controls(collected from Physical Examination Center from March 2014 to March 2015)was carried out. The genetypes and allele frequency were compared; the clinical data, lipoprotein oxidation level and NAD(P)H oxidase activity were performed correlation analysis between different patient groups and different genetypes. Results There were no differences in low-density lipoprotein(LDL), oxidized LDL and malondialdehyde levels, NAD(P)H oxidase activity, and frequencies of CT+TT genotype and T allelic gene between patients with hyperlipidemia and controls(P>0.05). Group of cerebral infarction had significantly higher serum levels of oxidized high-density lipoprotein(HDL)and malondialdehyde, and NAD(P)H oxidase activity than the controls(P 0.05), but CC genotype patients had significantly higher serum levels of oxidized HDL, oxidized LDL and malondialdehyde, and NADPH oxidase activity than CT+ TT genetype patients(P<0.05). Logistic regression analysis indicated that smoking, hypertension, serum levels of lipoprotein(a), oxidized HDL and malondialdehyde, NADPH oxidase activity and T allelic gene(OR=0.299, 95% CI: 0.102-1.879, P=0.028)were independent risk factors for cerebral infarction. Conclusion C242T polymorphism is associated with lipoprotein oxidation and cerebral infarction in Han people from Shanghai Songjiang of China, and T allelic gene and lipoprotein oxidation are independent risk factors for cerebral infarction. Key words: Nicotinamide adenine dinucleotide phosphate oxidase; C242T polymorphism; Lipoprotein oxidation; Hyperlipidemia; Cerebral infarction