Abstract
Experimental autoimmune encephalomyelitis (EAE) is a murine model of multiple sclerosis (MS). Current MS treatment consists in general suppression of immune response which increases susceptibility to infections. Considering the immunomodulatory properties and tolerogenic effects of active vitamin D, this study aimed to evaluate the therapeutic effect of myelin oligodendrocyte glycoprotein (MOG) associated with vitamin D in EAE development. Female C57BL/6 mice were submitted to EAE induction by immunization with MOG emulsified with Complete Freund Adjuvant plus Mycobacterium tuberculosis. Two intraperitoneal doses of Bordetella pertussis toxin were also injected. One day after immunization, mice were treated with 0,1mug of vitamin D every other day during 15 days (on days 1, 3, 5, 7, 9, 11, 13 and 15). MOG (150mug) was co‐administered on days 3 and 11. The administration of vitamin D or MOG alone determined significant reduction in EAE incidence and in clinical scores. When MOG was associated with vitamin D the animals (n=12) did not develop clinical disease. In this group there was also a decreased production of IL‐17 and IL‐6 by spleen and CNS mononuclear infiltrating cells stimulated with MOG, in comparison to the EAE control. In addition, this treatment inhibited dendritic cells (CD11c+MHC‐II+CD86+) maturation in the spleen. In conclusion, the association of MOG with vitamin D was able to control EAE development.Grant Funding Source: FAPESP
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