Association of miR-502-Binding Site Single Nucleotide Polymorphism of SET8 Gene with Non-Small Cell Lung Cancer Risk in Chinese People

Abstract

Genetic polymorphisms in 3 � -untranslated regions (3 � -UTRs) targeted by miRNAs alter the strength of miRNA binding, with consequences on regulation of target genes thereby affecting the individual’s cancer risk. This study was aimed at investigating the association between the gene polymorphism (rs16917496 C/T) within the miR-502-binding site in the 3 � -UTR of the SET8 and non-small cell lung cancer (NSCLC) risk in Chinese population. The genotypes of SET8 polymorphism of peripheral blood DNA were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct DNA sequencing in a case-control study on 120 NSCLC cases and 164 controls. The CC genotype was associated with a significant decreased risk of NSCLC (P = 0� 011; OR = 0� 307, 95% CI = 0� 119–0.793) by comparing of the TT genotype. The carriers of C allele at the 3 � -UTR polymorphism showed a significantly reduced NSCLC risk (P = 0� 041; OR = 0� 674, 95% CI = 0� 461–0.986) as compared with non-carriers. Therefore, the miR-502-binding site single nucleotide polymorphism (SNP rs 16917496) within the SET8 gene may be a marker of reduced genetic susceptibility to NSCLC in Chinese population.