Association of miR-21, miR-126 and miR-605 gene polymorphisms with ischemic stroke risk.

Yang Xiang,Ye-Sheng Wei,Hua-Tuo Huang,Hong-Cheng Luo,Tan Tan,Jing Guo,You-Fan Peng

doi:10.18632/oncotarget.21316

Yang Xiang, Ye-Sheng Wei + Show 5 more

Open Access

https://doi.org/10.18632/oncotarget.21316

Copy DOI

Abstract

We investigated whether three common microRNA polymorphisms (miR-21T>C [rs1292037], miR-126G>A [rs4636297] and miR-605T>C [rs2043556]) were associated with ischemic stroke (IS) risk in a Chinese population. The study population comprised 592 ischemic stroke patients and 456 normal controls. The polymorphisms were measured using Snapshot SNP genotyping assays and confirmed by sequencing. Relative expressions of miR-21, miR-126 and miR-605 were measured by quantitative real-time PCR. We found that miR-126 gene rs4636297 polymorphism was associated with decreased ischemic stroke risk (GA vs. GG: AOR=0.64, adjust P=0.025; AA vs. GG: AOR=0.32, adjust P=0.007; dominant model: AOR=0.58, adjust P=0.004). MiR-21 gene rs1292037 and miR-605 gene rs2043556 polymorphisms were not associated with ischemic stroke risk. In addition, compared with normal controls, serum miR-126 level was significantly decreased in ischemic stroke patients, while the miR-21 level was significantly increased. Importantly, patients carrying rs4636297 GA/AA genotypes had higher serum miR-126 level (P<0.05). MiR-126 gene rs4636297 polymorphism and serum miR-126/-21 levels are associated with ischemic stroke risk. Our data indicates that miR-126 and miR-21 play roles in the development of ischemic stroke.

Highlights

Ischemic stroke is a common cerebral blood circulation disorder disease which is a serious threat to human health
Compared with rs4636297 GG genotype, rs4636297 GA and AA genotypes were significantly associated with decreased risk of ischemic stroke
We demonstrated for the first time that miR-126 gene rs4636297G>A polymorphism was associated with a reduced risk of ischemic stroke

Summary

Introduction

Ischemic stroke is a common cerebral blood circulation disorder disease which is a serious threat to human health. It is the second leading cause of death in China [1]. Its growing trend of prevalence deserves the attention of medical community worldwide Common risk factors, such as age, gender, smoking, hypertension and abnormal lipid metabolism account for part of the prevalence of ischemic stroke [2,3,4,5]. MicroRNAs (miRNAs) are class of small noncoding single-stranded RNAs molecules of about 21-23 nucleotides in length, which involve in regulating gene expressions and contribute to various disease pathogenesis, such as inflammation, cancer, atherosclerosis, and ischemic stroke [9,10,11,12,13]. The exact mechanism of the abnormal miR-21 and miR-126 expression remain unclear and genetic factors may play key roles in this process [21]

Methods

Results

Conclusion