Association of miR-146a, miR-149, miR-196a2, miR-499 gene polymorphisms with ischemic stroke in a Chinese people.

Hong-Cheng Luo,Chun-Fang Wang,Bei-Lin Li,Ye-Sheng Wei,Qi-Sheng Luo,Ming Lei

doi:10.18632/oncotarget.18333

Hong-Cheng Luo, Chun-Fang Wang + Show 4 more

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https://doi.org/10.18632/oncotarget.18333

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Abstract

This study aimed to investigate genetic polymorphisms of miR-146a, miR-149, miR-196a2, and miR-499 and genetic susceptibility of ischemic stroke in the population of Guangxi in China. A case–control study was used to investigate miRNAs genetic polymorphisms in 298 patients with ischemic stroke and 303 healthy controls. Single-base extension polymerase chain reaction genotyping principle was used to detect genetic polymorphisms of miRNAs,and the relationship of genotype in each group and blood lipid was compared and analyzed. The genetic polymorphism of miR-499A>G (rs3746444) was associated with ischemic stroke (P < 0.05), and the risk of ischemic stroke was high in patients with G allele (OR = 1.455; 95% CI = 0.531–2.381; P = 0.039) and AG (OR = 1.339; 95% CI = 1.126–1.967; P = 0.037) genotype. The levels of low-density lipoprotein cholesterol, very-low-density lipoprotein cholesterol, homocysteine, and lipoprotein in the ischemic stroke group were higher than those in the control group (P < 0.05). The genetic polymorphism of miR-499A>G (rs3746444) was related to ischemic stroke, and G allele and AG genotype may increase the risk of ischemic stroke in the population of Guangxi in China.

Highlights

Ischemic stroke (IS) is a cardiovascular disease that seriously threatens the health of middle-aged and elderly people
The genetic polymorphism of miR499A>G was associated with ischemic stroke (P < 0.05), and the risk of ischemic stroke was high in patients with G allele (OR = 1.455; 95% CI = 0.531–2.381; P = 0.039) and AG (OR = 1.339; 95% CI = 1.126–1.967; P = 0.037) genotype
Three genotypes were detected by Single nucleotide polymorphism (SNP) of miR146aC>G gene, including GG, GC, and CC (Figure 1); miR-149T>C gene had three genotypes, including CC, computed tomography (CT), and TT (Figure 2)

Summary

Introduction

Ischemic stroke (IS) is a cardiovascular disease that seriously threatens the health of middle-aged and elderly people. In China, due to its high morbidity, high disability rate, high mortality, and high recurrence rate, IS has surpassed the morbidity of heart disease and tumors [1], which brings tremendous economic pressure and health burden to the family and the society [2,3,4]. Hypertension, diabetes, hyperlipidemia, and high homocysteine content are risk factors for IS [5]. IS is a complex disease induced by the combined action of a variety of genetic and environmental factors, and genetic mutations play a crucial role in its development [6,7,8].

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