Association of miR-146a, miR-149 and miR-196a2 polymorphisms with neuroblastoma risk in Eastern Chinese population: a three-center case-control study.

Chunlei Zhou,Jing He,Haixia Zhou,Haiyan Wu,Yingzi Tang,Jinhong Zhu,Lili He,Yizhen Wang,Jinghang Li

doi:10.1042/bsr20181907

Abstract

Neuroblastoma is one of the most common malignancy in childhood, which originates from the developing sympathetic nervous system. Single nucleotide polymorphisms (SNPs) in primary miRNA (pri-miRNA) have shown to associate with cancer susceptibility, including neuroblastoma. Three precursor miRNA (pre-miRNA) SNPs (pre-miR-146a rs2910164, pre-miR-149 rs2292832 and pre-miR-196a2 rs11614913) were found to contribute to pathogenesis of various diseases. Here, to evaluate the association among these three pre-miRNA SNPs and neuroblastoma susceptibility in Eastern Chinese children, we carried out a three-center case–control study involving 312 neuroblastoma cases and 762 healthy controls. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the association of these three polymorphisms with neuroblastoma risk. However, no significant association was observed among these three SNPs and neuroblastoma susceptibility, in either overall or subgroups analysis by tumor sites, gender and age. Further larger studies consisting of diverse ethnic populations are required to clarify the associations among these three pre-miRNAs polymorphisms and neuroblastoma risk.

Highlights

Neuroblastoma, a common childhood extracranial tumor, originates from the developing sympathetic nervous system [1]
We performed a three-center hospital-based case–control study to test the association among these three pre-miRNAs’ single nucleotide polymorphism (SNP) and neuroblastoma risk with 312 cases and 762 controls residing in East China
No significant association was observed among the three selected SNPs and neuroblastoma risk in the combined study population

Summary

Introduction

Neuroblastoma, a common childhood extracranial tumor, originates from the developing sympathetic nervous system [1]. The single nucleotide polymorphisms (SNPs) in precursor miRNA (pre-miRNA) encoding sequences may influence miRNA expression and maturation, thereby modifying susceptibility to neuroblastoma and other cancers [22,23,24,25,26]. Previous studies showed that miR-146a was related to regulation of TNF-α [30], miR-149 could regulate the expression of MTHFR [31], and miR-196a2 could target ANXA1, DFFA and PDCD4 [32] These three well-known pre-miRNAs polymorphisms have been reported to be associated with the pathogenesis of various diseases, such as breast cancer, colorectal cancer, hepatocellular carcinoma, and gastric cancer [33,34,35,36,37,38]. We performed a three-center hospital-based case–control study to test the association among these three pre-miRNAs’ SNPs and neuroblastoma risk with 312 cases and 762 controls residing in East China

Materials and methods

Results

Discussion