Association of Metallothionein 1A gene polymorphisms at rs11640851 and rs8052394 with risk of type 2 diabetes mellitus in Indian population

Abstract

BackgroundMetallothioneins (MTs) are cysteine rich metal binding proteins that protect the cell proteins from oxidative damages. The studies have shown ambiguous findings regarding the association of SNPs in MT1A gene with type 2 diabetes mellitus (T2DM), therefore the present study was conducted to investigate the relationship of two SNPs (rs11640851 and rs8052394) of MT1A gene and haplotypes derived from these SNPs with T2DM in North Indians. MethodsThe present case-control study, conducted from August 2017 to July 2020, involved 168 T2DM patients and 102 healthy controls. SNPs rs11640851 and rs8052394 were genotyped from genomic DNA in the studied samples using PCR-RFLP method. ResultOut of two SNPs, only one SNP rs11640851 (+647 A/C) was found to be associated with T2DM and frequency of C allele was found to be higher in T2DM subjects (C vs A, OR = 1.58, 95% CI 1.07–2.33, p = 0.02). Among the four haplotypes (A/A, A/G, C/A, C/G), only C/A haplotype was found to be significantly associated with increased T2DM risk (C/A, OR = 1.97, CI 1.28–3.04, p = 0.001). ConclusionIt can be concluded from these results that MT1A gene polymorphism at SNP rs11640851 was associated with T2DM and C allele may be a predisposing factor in the development of T2DM.