Abstract
Increasing evidence has demonstrated the association between long noncoding RNAs (lncRNAs) and multiple autoimmune diseases. To explore four lncRNAs (GAS5, lnc-DC, linc0597 and linc0949) expression levels and gene polymorphisms in systemic lupus erythematosus (SLE), a two stage design was applied. In the first stage, 85 SLE patients and 71 healthy controls were enrolled to investigate the lncRNAs expression levels. Then, 1260 SLE patients and 1231 healthy controls were included to detect the single nucleotide polymorphisms (SNPs) in the differentially expressed lncRNAs identified in the first stage. Linc0597, lnc-DC and GAS5 expression levels were significantly lower in SLE patients than healthy controls (P < 0.001, P < 0.001, P = 0.003 respectively). Association of five SNPs (rs10515177, rs2070107, rs2632516, rs2877877, rs2067079) with SLE risk were analyzed. No significant association was observed between these gene polymorphisms and susceptibility to SLE (all P > 0.010), and we did not find significant association between any genotypes at five SNPs and their respective lncRNAs expression in SLE (all P > 0.010). In summary, the expression levels of linc0597, lnc-DC and GAS5 are decreased in SLE patients, but their gene polymorphisms are not associated with SLE risk, and do not influence their expression levels.
Highlights
Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune disease which is characterized by multiple autoantibody production, formation of immune complexes that result in multiple tissue or organ damages[1,2,3]
We aimed to investigate the expression levels of these long noncoding RNAs (lncRNAs) in peripheral blood mononuclear cells (PBMCs) from systemic lupus erythematosus (SLE) patients and healthy controls, as well as the association of their gene polymorphisms with susceptibility to SLE and their expression levels
The four lncRNAs (GAS5, lnc-dendritic cells (DCs), linc0597 and linc0949) expression levels in PBMCs from 85 patients with SLE and 71 healthy controls were shown in Table 1, Fig. 1
Summary
Systemic lupus erythematosus (SLE) is a chronic multisystem autoimmune disease which is characterized by multiple autoantibody production, formation of immune complexes that result in multiple tissue or organ damages[1,2,3]. The accurate functions of lncRNAs remains largely unclear, a number of studies have revealed that lncRNAs participate in various critical biological processes, such as chromatin remodeling, gene transcription, RNA splicing, and protein transport diverse mechanisms[10,11,12], implicating their role in a wide range of complex human diseases[13,14]. Wang et al.[24] identified a kind of lncRNA, lnc-DC, which was exclusively expressed in human conventional DCs, and regulated DCs differentiation to stimulate T cell activation. Based on the available evidence and our recent study on the plasma expression of lncRNAs25, we hypothesized that GAS5, lnc-DC, linc0597 (BZRAP1-AS1) and linc0949 (OIP5-AS1) may play a critical role in the pathogenesis of SLE. We aimed to investigate the expression levels of these lncRNAs in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls, as well as the association of their gene polymorphisms with susceptibility to SLE and their expression levels
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