Association of Lipoprotein Lipase Gene Polymorphism and Coronary Artery Disease in Korean

Abstract

Background:The accumulation of lipoprotein and monocyte in the intima of the arterial wall is the most important step of the development of coronary artery disease (CAD). Lipoprotein lipase (LPL) plays an antiatherogenic role by lipolysis of triglyceride-rich lipoproteins, but, it may also act as a receptor of some lipoproteins and monocyte at the arterial wall and act as a atherogenic molecule. Previous studies showed somewhat contradictory results about the association of CAD and LPL polymorphisms and mutations. Racial and dietary difference may contribute to these contradictory results. In this study, we tried to find out the association of CAD and the genetic variation of the LPL (PvuII RFLP in intron 6, HindIII RFLP in intron 8 and Ser 447 Ter mutation in exon 9) in Korean population. Method and Result:CAD patients (n=146), confirmed by coronary angiography and healthy Korean adult volunteers (n=110) were genotyped for PvuII /HindIII RFLP and Ser447Ter mutation of the LPL gene by PCR-digestion method. Between two groups, the genotype frequency of these genetic variations was not different. But, the genetic variations showed different effect on lipid profile and body mass index (BMI) in the CAD group and in the control group. In the CAD group, P1 allele carriers showed higher total cholesterol (P1P1+P1P2:P2P2=216±51 mg/dl:198±38 mg/dl, p=0.039) and higher LDL cholesterol level (P1P1+P1P2:P2P2=143±46 mg/dl:126±36 mg/dl, p=0.047), and H1 allele carriers had lower Body mass index than non-carriers (23.8±2.3 kg/m:24.8±2.9 kg/m, p=0.047). In the control group, the Ser447Ter mutation carriers had higher HDL cholesterol level than non-carriers (59±10mg/dl versus 53±11mg/dl, p=0.049) and patients with P1 allele showed lower body mass index (P1P1+P1P2:P2P2=23.1±2.6 kg/m:24.5±2.6 kg/m, p=0.006). Conclusion:In Korean, PvuII/HindIII RFLP and Ser447Ter mutation was not associated with CAD, and they showed different effect on the lipid profile and on the body mass index according to the study group. These results 논문접수일:1999년 4월 6일 심사완료일:1999년 6월 9일 교신저자:김효수, 110-744 서울 종로구 연건동 28 서울대학교 의과대학 내과학교실 전화:(02) 760-2226·전송:(02) 766-8904 E-mail: hyosoo@plaza.snu.ac.kr