Abstract
BackgroundIncreasing evidence suggests that alterations in mitochondrial DNA (mtDNA) content may be implicated in the tumorigenesis of several malignancies. However, the association between mtDNA content in peripheral blood lymphocytes (PBLs) and glioma risk has not been investigated.MethodsReal-time PCR was used to examine the mtDNA content in PBLs of 414 glioma patients and 414 matched controls in a hospital-based case–control study. The association between mtDNA content and glioma risk was evaluated using an unconditional multivariate logistic regression model.ResultsWe found that glioma patients exhibited a significantly higher median mtDNA content than healthy controls (0.99 vs. 0.71, P < 0.001). Unconditional multivariate logistic regression analysis adjusting for age, gender, smoking status, and family cancer history showed that there was an S-shaped association between mtDNA content and glioma risk. Higher mtDNA content was significantly associated with an elevated risk of glioma. Compared with the first quartile, the odds ratio (95% confidence interval) for subjects in the second, third, and fourth quartiles of mtDNA content were 0.90 (0.52-1.53), 3.38 (2.15-5.31), and 5.81 (3.74-9.03), respectively (P for nonlinearity = 0.009). Stratified analysis showed that the association between mtDNA content and glioma risk was not modulated by major host characteristics.ConclusionsOur findings demonstrate for the first time that a higher mtDNA content in PBLs is associated with an elevated risk of glioma, which warrants further investigation in larger populations.
Highlights
Increasing evidence suggests that alterations in mitochondrial DNA content may be implicated in the tumorigenesis of several malignancies
Several studies have demonstrated that the alterations of mitochondrial DNA (mtDNA) content in peripheral blood lymphocytes (PBLs) can be used as a surrogate of constitutive genetic background to predict the risk of cancers such as renal cell carcinoma (RCC), breast cancer, lung cancer, non-Hodgkin lymphoma (NHL), and colorectal cancer (CRC) [15,16,17,18,19]
Further analysis indicated that no significant correlation was found between mtDNA content and levels of platelet or white blood cell types
Summary
Increasing evidence suggests that alterations in mitochondrial DNA (mtDNA) content may be implicated in the tumorigenesis of several malignancies. The association between mtDNA content in peripheral blood lymphocytes (PBLs) and glioma risk has not been investigated. MtDNA content in patient tissues has been found to be increased in cancers of head and neck, ovary and esophagus [5,9,10], and decreased in hepatocellular carcinoma (HCC), advanced gastric cancer, osteosarcoma, breast cancer and renal cell carcinoma (RCC) [8,11,12,13,14]. Several studies have demonstrated that the alterations of mtDNA content in peripheral blood lymphocytes (PBLs) can be used as a surrogate of constitutive genetic background to predict the risk of cancers such as RCC, breast cancer, lung cancer, non-Hodgkin lymphoma (NHL), and colorectal cancer (CRC) [15,16,17,18,19]. To date, the association between mtDNA content in PBLs and glioma susceptibility has not been determined
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