Association of laminin receptor gene fragments with nuclear matrices in high and low metastatic cancer cell lines

Abstract

Interactions between tumor cells and laminin play an important role in the complex cascade of events in metastasis. We investigated the association of laminin receptor DNA fragments with nuclear matrix in two carcinoma cell lines with different metastatic potentials. Two fragments of DNA (474 bp and 855 bp) were amplified and detected in the genomic DNA of both cell lines. With the extraction of residual DNA from nuclear matrix, positive association of the 474 bp DNA fragment with nuclear matrix was demonstrated in both cell lines. Nevertheless, the intensity of this amplified product in the high metastatic cell line is much stronger than that in the low metastatic cell line. The long DNA fragment was not detected in both cell lines indicating that part of the laminin receptor gene was hardly intact after sequential extraction with detergent, high salt and enzymes. Our findings show the possibility that the attachment of the 474 bp DNA fragment to nuclear matrix may contribute to the difference in metastatic properties of these two cell lines.