Abstract
Kv10.1 is voltage gated potassium channel of the EAG family, which is mainly expressed in the Central Nervous System (CNS) under physiological conditions. In the past years, a causal association between Kv10.1 and tumor growth has also been proven, nevertheless the precise mechanism by which this occurs remains unknown. In this work we compare the direct association of membrane-associated proteins to Kv10.1 in different membrane domains of brain and cancer cells. Plasma membranes of brain tissue of adult mice (C57BL/6) and of the human prostate cancer cell line DU-145 were isolated. Detergent resistant membranes (DRM) were extracted through Triton X-100 solubilization and discontinuous gradient centrifugation. IP-assay for Kv10.1 was performed in the different membrane domains; protein expression was analyzed through the Colloidal Coomasie method. Kv10.1 associates partially to the DRM in brain tissue whereas in cancer cells no association can be observed. The protein pattern associated to Kv10.1 in the different systems also varies. We conclude that the different domain association of Kv10.1 is important for different protein interactions and could be involved in it's role as a tumor promoting protein.
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