Association of IL-1β, IL-6, TNF-α, and TGFβ1 Gene Polymorphisms with Recurrent Spontaneous Abortion in Polycystic Ovary Syndrome.

Afrah F Alkhuriji,Lamjed A Mansour,Suliman Y Al Omar,Zainb A Babay,Wazirah G Alharbi,Manal F El-Khadragy,Mahmoud I Khalil

doi:10.1155/2020/6076274

Abstract

Recurrent spontaneous abortion (RSA) is a common pregnancy-associated complication of polycystic ovary syndrome (PCOS) which is an endocrine malfunction disease. Patients with PCOS may have several underlying contributing and interrelated factors, which have been reported in women with RSA. The incidence rate between PCOS and RSA remains uncertain. The aim of this study is to determine the possible association of IL-1β-511C/T, IL-6-174G/C, TNF-α-1031T/C, and TGFβ1-509T/C with RSA patients with or without PCOS. A total of 140 RSA patients, 70 of which were PCOS patients, and 140 healthy females with no history of RSA or PCOS were included in this study. PCR amplification, genotyping, and sequence analysis were employed to investigate the presence of the polymorphisms. The genotypic and allelic frequencies were calculated separately for each subject. Out of the four studied polymorphisms, the IL-1β-511C/T genotype in RSA without PCOS patients (12.7%) was significantly different compared with that in control subjects (p = 0.047). For IL-6-174C/G, there was a tendency towards more CC carriers among RSA with PCOS patients (10%) than in controls (3%). The GG genotype in RSA women with PCOS (60%) was significantly different compared with that in control subjects (p = 0.033), and the GC genotype in RSA with PCOS patients (30%) showed a marginal significant difference compared with that in control subjects (p = 0.050). Significant difference was identified in the allelic frequencies in RSA patients with PCOS compared to controls (p = 0.025). IL-6-174G/C and TNF-α-1031T/C polymorphisms are significantly associated with RSA patients in Saudi patients with PCOS, while the IL-1β-511C/T polymorphism is significantly associated with RSA patients without PCOS.

Highlights

Recurrent spontaneous abortion (RSA) is a common pregnancy-associated complication
The analysis of genotype and allele distribution of IL-1β511C/T, IL-6-174G/C, TNF-α-1031T/ C, and TGFβ1-509T/C gene polymorphisms was performed on all subjects (Table 3)
The genotypes of TNF-α-1031T/C did not differ significantly between the RSA patients with or without Polycystic ovary syndrome (PCOS) and healthy controls, significant difference was identified in the allelic frequency in RSA patients with PCOS compared to controls

Summary

Introduction

Recurrent spontaneous abortion (RSA) is a common pregnancy-associated complication It is defined as three or more consecutive pregnancy losses that occur prior to 24 gestational weeks at a rate of 15-20% and affecting about 2-4% of couples [1, 2]. Disease Markers thrombophilias, immunological abnormalities, endocrine issues, obesity, infections, parental age, and environmental factors. Polycystic ovary syndrome (PCOS) is an endocrine malfunction disease, characterized by chronic anovulation and hyperandrogenism [5]. In 2003, a joint European Society of Human Reproduction and Embryology and American Society for Reproductive Medicine (ESHRE/ASRM) consensus meeting produced a refined definition of PCOS, namely, the presence of two out of three criteria: (1) oligo- and/or anovulation; (2) hyperandrogenism (clinical and/or biochemical); and (3) polycystic ovaries, with the exclusion of other etiologies [6, 7]. Polymorphisms, and differential regulation of genes may contribute to the pathogenesis of PCOS [10]

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