Abstract
The association of IGF-I gene polymorphisms with certain traits in 708 individuals of two Chinese dairy-goat breeds (Guanzhong and Xinong Saanen) was investigated. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variation. Two novel mutations were detected in the 5'-flanking region and in intron 4 of IGF-I gene, viz., g.1617 G > A and g.5752 G > C (accession D26119.2), respectively. The associations of the g.1617 G > A mutation with milk yield and the body size were not significant (p > 0.05). However, in the case of g.5752 G > C, Xinong Saanen dairy goats with the CG genotype presented longer bodies (p < 0.05). Chest circumference (p < 0.05) was larger in Guanzhong goats with the GG genotype. In Xinong Saanen dairy goats with the CC genotype, milk yields were significantly higher during the first and second lactations (p < 0.05). Hence, the g.5752 G > C mutation could facilitate association analysis and serve as a genetic marker for Chinese dairy-goat breeding and genetics.
Highlights
Animal growth is controlled by a complex system, in which the somatotropic axis plays a key role
Genes that operate in this axis are responsible for postnatal growth, mainly by way of growth hormones acting on bones and muscles mediated by insulin-like growth factor I (IGF-I) (Sellier, 2000)
The IGF-I gene was mapped on chromosome 5 in bovine (Miller et al, 1991)
Summary
Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have