Association of HOTAIR (rs920778 and rs1899663) and NME1 (rs16949649 and rs2302254) gene polymorphisms with breast cancer risk in India

Abstract

Until now, no study has reported the combined effect of genetic variants of HOTAIR and NME1 towards breast cancer (BC) pathogenesis. Hence, the aim of the present study is to determine the risk of breast cancer development with HOTAIR (rs920778 C>T and rs1899663 G>T) and NME1 (rs16949649 T>C and rs2302254 C>T) genetic polymorphisms in the Indian population for the first time. To investigate the genetic association of these four SNPs, we conducted a population-based case-control study involving 1011 subjects (502 histologically confirmed BC patients and 509 disease-free controls) using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. HOTAIR rs920778 TC genotype elevated the risk of BC (OR=1.39, 95% CI=1.06-1.83, p=0.018) and individuals carrying the mutant allele (T) of rs1899663 had increased BC risk (OR=1.23, 95% CI=1.02-1.47, p=0.026). The presence of the NME1 rs16949649 CC genotype increased the risk of BC (OR=1.76, 95% CI=1.15-2.71, p=0.009). Moreover, the HOTAIR rs920778 variant (TC+CC) increased the risk of BC in pre-menopausal women (OR=5.86; p<0.0001). Women carrying 2 or 3 mutant alleles for the investigated SNPs were observed to have an elevated risk of BC. The results of the present study highlight the presence of significant associations between NME1 rs16949649 and HOTAIR (rs920778 and rs1899663) polymorphisms and breast cancer development in Indian women.