ASSOCIATION OF HIF-1 ALPHA AND BETA SUBUNITS WITH PER1 AND CRY1 PROTEINS IN OBSTRUCTIVE SLEEP APNEA PATIENTS

Abstract

TYPE: Abstract Publication TOPIC: Sleep Disorders PURPOSE: The aim of the study was to assess the relationship between HIF-1alpha, HIF-1beta, PER1, CRY1, and polysomnography (PSG) variables in healthy individuals and severe OSA patients. METHODS: The study included 20 individuals, who underwent PSG and based on apnea-hypopnea index (AHI) were divided into severe OSA group (n=10; AHI>=30; 90% male) and healthy control (n=10; AHI<5; 70% male). All participants had their peripheral blood collected in the evening (9:00-10:00 pm) before and in the morning (6:00-7:00 am) after the PSG. HIF-1alpha, HIF-1beta, PER1 and BMAL1 protein concertation measurements were performed using ELISA. The study was financed by Polish National Centre Grant no. 2018/31/N/NZ5/03931. RESULTS: Significant difference was observed in the following protein measurements between study groups: evening and morning HIF-1alpha (p=0.020 and p=0.043, respectively), evening HIF-1beta (p=0.047), evening and morning PER1 (p=0.004 and p=0.029, respectively) and evening CRY1 (p=0.035). No differences were observed between morning and evening protein levels in both groups. Evening HIF-1alpha correlated with evening and morning PER1 (R=0.618, p=0.004 and R=0.514, p=0.020, respectively) as well as morning CRY1 (R=0.468 p=0.038), while morning HIF-1beta with evening and morning PER1 (R=0.629, p=0.003 and R=0.471, p=0.036, respectively). CONCLUSIONS: There are significant differences in circadian clock proteins (PER1 and CRY2) concentration in OSA and control group, which suggests that OSA patients are in risk of circadian clock disruption, which might be mediated by HIF-1 subunits. CLINICAL IMPLICATIONS: OSA patients might suffer from additional circadian disruption caused by present during the night hypoxia. DISCLOSURE: No significant relationships. KEYWORDS: hypoxia, circadian clock, OSA