Association of GSTM1, P1, T1 and CYP2E1 Single Nucleotide Polymorphisms with Colorectal Cancer in Iran

Abstract

Purpose: Colorectal cancer rate is very high and common cause of death. Mortality rate from colorectal cancer can be reduced by identification and removal of cancer precursors, adenomas, or by detection of cancer at an earlier stage. We performed this study to determine the association between genetic polymorphisms of cytochrome P4502E1 (CYP2E1) and glutathione S-transferases P1, M1 and T1 (GSTP1, M1, T1) and susceptibility to colorectal cancer (CRC). Methods: Genotyping of CYP2E1 and GSTP1, M1, T1 was performed by using pyrosequencing analysis on DNA isolated from blood sample from colorectal cancer cases (N = 100) and normal controls (N = 100). Results: Mean GSTT1 activity was significantly (P < 0.01) higher in cases as campared to controls (P < 0.0001; OR, 2.43; 95% CI, 1.47–4) on the other hand there is no significant association between GSTM1, P1, CYP2E1 and colorectal cancer. Conclusion: GSTT1 measurement may be useful as a colorectal marker in colorectal cancer and biopsies obtained at colonoscopy can be used to measure tumor markers.Table: The genotype frequencies of GSTM1, P1, T1 and CYP2E1 polymorphisms