Association of GH Gene Polymorphism with Semen Parameters of Boars

M Kmieć,H Wierzbicki,A Terman,S Zych

doi:10.2754/avb200776010041

M Kmieć, H Wierzbicki + Show 2 more

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https://doi.org/10.2754/avb200776010041

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Abstract

Relations between polymorphism of the Growth Hormone gene and semen characters were analyzed. The DNA for the purpose of examination was isolated from the peripheral blood of 173 boars. In the boar herd under study the frequency of allele occurrence for the GH/MspI was as follows: allele GHA - 0.79 and allele GHB - 0.21. As far as the GH/HaeII polymorphism is concerned, the relevant frequency was as follows: allele GHA - 0.53 and allele GHB - 0.47, respectively. The relationship between the GH genotypes and semen characteristic traits were analyzed. The study showed that boars with GHBGHB genotype of both polymorphous loci of the GH gene produced ejaculates of larger volume, higher percentage, number of normozosperms in the ejaculate and number of insemination as compared to GHA GHA and GHAGHB boars. Our current findings suggested that polymorphism of the GH/MspI and GH/HaeII might have potential effect for reproductive performance traits of boars.

Highlights

Relations between polymorphism of the Growth Hormone gene and semen characters were analyzed
After the digestion with endonuclease MspI, that identified sequences 5’CZCGG-3’ and 3’-GGC-C-5’ in the second intron of GH gene various arrays of bands were found in the agarose gel and two alleles GHA and GHB were found that affect the occurrence of three genotypes (GHAGHA - 222, 147 and 137 bp; GHAGHB - 284, 222, 147 and 137 bp and GHBGHB - 284 and 222 bp compared to DNA pUC19/MspI molecular marker)
The amplified product of 506 bp was subject to the digestion with endonuclease HaeII, that identified sequences 5’-RGCGCZY↓ 3’ and 3’-Y↑CGCGR-5’ in the second exon of GH gene and various arrays of strips were found in the agarose gel and two alleles GHA and GHB were found that affect the occurrence of three genotypes (GHAGHA - 337 and 173; GHAGHB - 506, 333 and 173 bp and GHBGHB - 506 bp compared to DNA pUC19/MspI molecular marker)

Summary

Introduction

Relations between polymorphism of the Growth Hormone gene and semen characters were analyzed. In the boar herd under study the frequency of allele occurrence for the GH/MspI was as follows: allele GHA - 0.79 and allele GHB - 0.21. Results of the study showed that the GH directly affects the steroidogenesis, gametogenesis and gonad differentiation (Zachman 1992; Franks 1998). It plays a major role in the growth and development of testis since the discovery of the relation between the GH deficiency and male hypogonadism in humans (Spiteri-Grech and Nieschlag 1992). The testis performance and steroidogenesis and gametogenesis could simultaneously be affected by testicular and hypophyseal growth hormones (Hull and Harvey 2000). The genetic polymorphism of the GH gene in pigs was found and about 20 genetic variations of the GH gene in different breeds and lines of pigs were described

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Conclusion