Association of genetic polymorphisms of IL1β -511 C>T, IL1RN VNTR 86bp, IL6 -174 G>C, IL10 -819 C>T and TNFα -308 G>A, involved in symptomatic patients with dengue in Brazil.

Abstract

In this study, single nucleotide polymorphisms (SNP) of interleukin (IL) 1β -511C>T, IL1RN VNTR 86bp, IL6 -174G>C, IL10 -819C>T and TNFα -308G>A were analyzed by PCR-RFLP with symptoms of dengue with the clinical features. 196 individuals admitted to the São José Infectious Diseases Hospital with suspected dengue infection. Dengue was confirmed in 111 of the patients. The control group consisted of 85 other individuals confirmed without dengue. It was demonstrated that the presence the T allele of IL1β (P<0.05) was associated with susceptibility to developing the disease. Other results also suggested that the polymorphism in the combinations IL6×IL1β (C and T alleles, respectively), IL1β (T allele)×IL1RN (*2/*2 genotype), IL6 (C allele)×TNFα (A allele), IL10 (C/T genotype)×TNFα (A/A genotype) (P<0.01, P=0.01, P<0.05 and P=0.03, respectively) were associated with predisposition to developing the disease and its symptoms. In summary, the findings of this study in a Brazilian population point out the importance of studies of combinations of polymorphisms in the development of dengue, which can increase the risk of dengue infection and its severity.