Association of genetic polymorphisms in Sipal with circulating tumor cells in patients with breast cancer

Abstract

Objective To investigate the correlation between the polymorphisms (rs931127 and rs746429) in signal-induced proliferation-associated 1 ( Sipal ) gene and circulating tumor cells in patients with breast cancer.Methods The polymorphisms of Sipal were detected by using polymerase chain reaction (PCR)-DNA sequencing in 124 primary breast cancer patients and 120 healthy controls.The circulating tumor cells were detected by real-time quantitative PCR.Results The polymorphisms were not associated with the circulating tumor cells of the breast cancer patients ( P ＞ 0.05 ).rs931127 and rs746429 polymorphisms of Sipal were not significantly associated with age,estrogen receptor status,progesterone receptor status,and C-erbB2 overexpression of patients with breast cancer (P ＞ 0.05). However,patients with the rs931127 homozygous for the wild-type GG genotype were less likely to have positive lymph node involvement (22.6％) than those with heterozygous GA (48.3％) and mutant AA genotype (71.0％)(P ＜ 0.01 ).Patients with the rs746429 homozygous for the wild-type CC genotype were less likely to have positive lymph node involvement (31.4％) than patients with heterozygous CT (64.2％ ) and mutant TT genotype too (44.4％ ) ( P ＜ 0.01 ).Conclusion The variants rs746429 and the rs931127 allele were not associated with the circulating tumor cells of the breast cancer patients,but associated with lymph node involvement.rs931127 A allele and rs746429 T allele in patients with breast cancer may be associated with lymphatic metastasis. Key words: Breast cancer; Metastasis; Single nucleotide polymorphism; Sipa1