Association of gene polymorphism with menorrhagia combined with thyroid pathology

O A Andriets O A Andriets,O M Yuzko O M Yuzko,Y V Tsysar Y V Tsysar

doi:10.18370/2309-4117.2020.56.73-77

O A Andriets O A Andriets, O M Yuzko O M Yuzko + Show 1 more

Open Access

https://doi.org/10.18370/2309-4117.2020.56.73-77

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Abstract

Purpose of the study: to establish the frequency of alleles and genotypes of GP IIIa gene polymorphism in adolescent girls with uterine bleeding that is concomitant thyroid pathology and conduct a thorough genetic analysis of patients.Materials and methods. 70 teenage girls with puberty menorrhagia were surveyed. Study groups: I (main) – 30 teenage girls with puberty menorrhagia against the background of thyroid gland pathology, II group (comparison) – 40 teenage girls diagnosed with puberty menorrhagia. Control group consist of 25 almost healthy teenage girls. GP IIIa gene polymorphism (PLA1/PLA2) was studied once using PCR polymerase chain reaction.Results. A1/A2 polymorphism of the GP IIIa gene in adolescent girls with menorrhagia against the background of thyroid pathology indicates the likely prevailing frequency of individuals with “favorable” A1 allele over such with A2A2 genotype with and without pathology: in 12.3 and 9 times respectively. In adolescents without concomitant pathology A1A1 genotype was observed 11.7% more frequently than those with thyroid disease and 15.0% more often than in the control group. Distribution of genotypes of the GP IIIa gene polymorphous locus corresponded to the expected Hardy-Weinberg population balance, both in general and separately in the surveyed groups.Conclusions. In adolescents with menorrhagia without thyroid pathology A1A1 genotype occur 11.7% more frequently than in girls with thyroid diseases and 15.0% more often than in the control group. Relative frequency of A1A2- genotype is 9.2% in girls with menorrhagia and thyroid pathology, and A2A2 genotype by 2.5% over such in adolescents of comparison groups. Thus, risk factors for uterine bleeding with existing thyroid pathology in adolescent girls may depend on gene polymorphism.

Highlights

Studying the genetic prerequisite for the development of uterine bleeding in girls of puberty age under existing thyroid pathology and without concomitant pathology is one of the top tasks of pediatric gynecology [8, 9, 12]
STUDY RESULTS The frequency of alleles and A1A2 genotypes of the GP IIIa gene polymorphism was conducted in adolescents with menorrhagia, including thyroid pathology and in healthy teenage girls
It was found that the incidence of occurrence “wild” A1 allele of the GP IIIa gene in teenage girls with menorrhagia is 2.41 times greater than “mutant” A2 allele: 99 (70.7%) 41 (29.3%) cases of 140 allocated allies (χ2 = 9.64, p = 0.002)

Summary

Introduction

Studying the genetic prerequisite for the development of uterine bleeding in girls of puberty age under existing thyroid pathology and without concomitant pathology is one of the top tasks of pediatric gynecology [8, 9, 12]. Clinical interest is only a spot mutation in the 33rd position of the GP IIIa protein, which leads to the replacement of leucin (Leu) with prolin (Pro), which is the result of transversion T on C in the exon 2 of the GP IIIa gene in position 1565 [14, 15, 23]. This replacement leads to conformation changes of the N-terminal disulfide loop of GP IIIa, which relates to the fibrinogen binding site. Purpose of the study is to establish thyroid pathology the frequency of alleles and genotypes of the GP IIIa polymorphism gene in the structure of puberty menorrhagia in girls with concomitant thyroid pathology and to identify risk factors for puberty menorrhagia based on genetic analysis

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Results

Conclusion