ASSOCIATION OF G894T NOS3 GENE POLYMORPHISM WITH ATRIAL FIBRILLATION IN PATIENTS WITH ARTERIAL HYPERTENSION IN UZBEK POPULATION

Abstract

Objective: To estimate influence of G894T polymorphism of NOS3 gene on risk of developing AF with AH in Uzbekistan. Design and method: Study included 206 patients with AH I–III degree (JSC/JSC, 2018) of both sexes. Mean age of patients was 58.82 ± 11.91 years, the mean duration of hypertension was 10.9 ± 7.2 years. To verify AF, ECG Holter monitoring was performed using a Cardiospy monitor. Genotyping of G894T polymorphism of the NOS3 gene was performed using real-time PCR using gene-specific primers of allele-specific probes, followed by fluorescence detection of corresponding alleles. Results of all studies were considered statistically significant at p<0.05 Results: Relationship between G894T polymorphism of NOS3 gene and risk of developing AF, patients were divided into two groups: cases, n = 91 - with AF and controls, n = 115 - without AF. Among cases, following distribution of genotypes and alleles of G894T polymorphism of the NOS3 gene was revealed: GG genotype - determined in 52.7%, GT genotype - in 45.1%, TT genotype - 2.2%, p = 0.000. Allelic distribution showed predominance of carriage of the G allele: G allele - 75.3%, T allele - 24.7%, p = 0.000. Among controls, allelic distribution was with significant prevalence of the G allele: G allele in 75.7%, T allele in 24.3%, respectively, Xi = 119.035, p = 0. 000. Ratio of GG:GT:TT - genotypes was as follows: 60.0%: 31.3%: 8.7%, Xi = 68.426, p = 0.000. An analysis revealed protective effect of G allele G894T gene polymorphism on risk of developing AF with AH. Based on general model of inheritance, protective effect of GG genotype on risk of developing AF in hypertensive patients was found: general model of inheritance demonstrated significant accumulation of GG genotype among controls 60.0% (Xi2 = 6.72 P = 0.03; OR = 0.74, 95% CI 0.43-1.30), while GT genotype was less common in 31.3% and the TT genotype is even rarer in - 8.7%. Whereas in cases GG and GT genotypes were found in same ratio: 52.7%: 45.1%, respectively. Conclusions: Results obtained indicate significantly greater accumulation of GG genotype G894T gene polymorphism among AH without AF. Carriage of GG genotype G894T gene polymorphism is associated with low risk of developing AF with AH.