Abstract
Changes in intestinal microbiota have been linked to the development of diarrhea predominant irritable bowel syndrome (IBS-D). In order to better elucidate the relationship between intestinal microbiota changes and IBS-D, we compared fecal microbiota of IBS-D rats and healthy control using pyrosequencing of bacterial 16S rRNA gene targeted. Furthermore, we explored the effects of different traditional Chinese medicine (TCM) on intestinal microbiota of IBS-D in dose-dependent manner. Our results showed that there was no significant difference in fecal microbial community diversity among the healthy control group, IBS-D rats and IBS-D rats treated with traditional Chinese medicine, but the fecal microbial composition at different taxonomic levels have changed among these groups. Interestingly, the weight of IBS-D rats treated with moderate doses (13.4 g/kg) of TCM increased significantly, and the diarrhea-related symptoms improved significantly, which may be related to the enrichment in Deferribacteres, Proteobacteria, Tenericutes, Lachnospiraceae, and Ruminococcaceae and the reduction in Lactobacillus in fecal samples.
Highlights
Irritable bowel syndrome (IBS) is a clinically common gastrointestinal dysfunction, manifested by abdominal pain, abdominal discomfort, bowel habits, and stool abnormalities
The results showed that fecal water content in the medium dose group and B. licheniformis group was lower than that of other two groups (P < 0:05). This suggested that it is not the higher the concentration of traditional Chinese medicine (TCM), the better the effect in the treatment of irritable bowel syndrome (IBS-D), and medium dose of TCM is more effective in improving diarrhea symptom. These results indicated that TCM can improve IBS-Drelated symptoms when the drug concentration was within a certain range
When the treatment group is compared with the disease group, we found that Eubacterium_hallii, Faecalibacterium, Lactobacillus, Ruminococcaceae_UCG-004, and Jeotgalicoccus that increased in disease modeling were significantly depleted after treatment of medium doses of TCM while Lachnospiraceae and Anaeroplasma were enriched in treatment group (Figure 3(d), Wilcoxon’s test, P < 0:05)
Summary
Irritable bowel syndrome (IBS) is a clinically common gastrointestinal dysfunction, manifested by abdominal pain, abdominal discomfort, bowel habits, and stool abnormalities. Is the condition repeatedly delayed and difficult to recover and gastrointestinal symptoms such as sleep disturbance, depression, or chronic fatigue irritable syndrome may occur [1]. A subset of IBS-D accounts for nearly 33% of IBS and is often associated with rectal urgency, increased stool frequency, loose/watery stools, and abdominal distension [6]. Since IBS is a heterogeneous disease [7], differences in intestinal function between different IBS subgroups may further affect the composition of the intestinal microbiota
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