Association of Fas/APO-1 gene polymorphism with systemic lupus erythematosus in Japanese.

Abstract

This study was undertaken to investigate the possible association of Fas gene mutation(s) or polymorphism(s) with systemic lupus erythematosus (SLE) in Japanese. Screening for structural defects of the Fas gene was performed by using reverse transcriptase-polymerase chain reaction (RT-PCR)/single-strand conformation polymorphism (SSCP) analysis in 57 patients with SLE, followed by direct sequencing for the aberrantly migrating bands. The frequency of Fas polymorphism was determined by sequence-specific oligonucleotide probe (SSOP) hybridization in 82 SLE patients and 132 ethnically matched healthy individuals. We found a novel polymorphism at nucleotide 297 (T297C), which was linked to Fas polymorphism at nucleotide 416 (A416G). The 297C/416G genotype was present in four of the 132 (3.0%) healthy controls, none of whom was homozygous for the genotype. The allele frequency for 297C/416G in the controls was 1.5%. In contrast, 10 of the 82 (12.2%) SLE patients carried the 297C/416G allele, including one patient homozygous for the genotype. The allele frequency in SLE patients was 6.7%. The 297C/416G allele was significantly frequent in SLE patients (P = 0.01, chi2) with a relative risk of 5.00. As the polymorphism 297C/416G is silent at the amino acid level, it may affect the expression of Fas itself or be linked to a neighbouring genetic abnormality that is responsible for the pathogenesis of SLE.