Abstract
To evaluate the pattern of equiliberative nucleoside transporters in viral hepatitis responding and non-responding patients, to correlate the liver fibrosis stage with the pattern among the non-responders, and to correlate the equiliberative nucleoside transporter status with housekeeping hypoxanthine-guanine phosphoribosyltransferase gene. The comparative cross-sectional study was conducted at the Molecular Biology and Genetics Department, Liaquat University of Medical and Health Sciences, Jamshoro, Pakistan, from March to August 2018, and comprised adult hepatitis C virus patients of either gender who completed six months of treatment. They were assessed for response to therapy in terms of the presence of the viral load in their serum by using real time polymerase chain reaction, and divided into responder group A and non-responder group B. The groups were compared and correlation between equiliberative nucleoside transporter expressions and liver fibrosis was evaluated. Data was analysed using SPSS 23. Of the 80 patients, 33(41.3%) were males and 47(58.8%) were females. The overall mean age was 37.46±10.61 years. In terms of response to treatment, there were 40(50%) in each of the two groups. Mean post-treatment duration was 15.38±30.09 weeks. Age was not significantly different with respect to gender (p>0.05), but the age pattern was significantly different between the two groups (p<0.001). Also, non-responders had significant post-treatment duration compared to the responders (p<0.001). Hypoxanthine-guanine phosphoribosyltransferase gene showed no significant difference between the groups (p=0.144). Equiliberative nucleoside transporter was significantly down-regulated in the non-responders (p<0.001) and showed correlation with the degree of liver fibrosis (p<0.034) compared to the responders. There was a significant association between equiliberative nucleoside transporters and liver fibrosis stage in hepatitis C virus non-responding patients.
Highlights
Hepatitis C is one of the important blood-borne pathogens
Equiliberative nucleoside transporter was significantly down-regulated in the non-responders (p
There was a significant association between equiliberative nucleoside transporters and liver fibrosis stage in hepatitis C virus non-responding patients
Summary
Hepatitis C is one of the important blood-borne pathogens. According to the WorldHealth Organisation (WHO), approximately 3-4 million new cases are detected every year globally and about 120 to 130 million people are infected with hepatitis C virus (HCV); about 3% of the global population [1]. Hepatitis C is one of the important blood-borne pathogens. Health Organisation (WHO), approximately 3-4 million new cases are detected every year globally and about 120 to 130 million people are infected with hepatitis C virus (HCV); about 3% of the global population [1]. HCV is composed of 9.6-kb single-strand ribonucleic acid (RNA) genome with ‘5’ untranslated region (UTR) that acts as the interior site of entry of ribosomes. Various genotypes have been identified based on their characteristic geographic distribution and clinical course, including 1, 2, 3, 4, 5, 6 and 7, where 1, 2, 3 and 4 are most common genotypes. Liver fibrosis on the other hand is the main complication related to chronic viral hepatitis which results in cirrhosis and later raises the risk of HCC. Equiliberative nucleoside transporters (ENTs) are classified into 4 human isoforms (hENT1-4), each of them consists of transmembrane domains (TMDs) and large loops that are
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