Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome.

Yuksel Seckin,Yusuf Turkoz,Zeynep Aksungur,Yılmaz Bılgıc,Ali Yigit,Elif Yesilada,Harika Gozukara,Gonca Gulbay,Oguzhan Yildirim,Yasir Furkan Cagin

doi:10.1155/2016/2579626

Abstract

Background. There are no studies investigating the relationship between endothelial nitric oxide synthase (eNOS) gene polymorphisms and hepatorenal syndrome (HRS). Aim. The purpose of this study is to elucidate whether eNOS gene polymorphisms (G894T and T-786C) play a role in the development of type-2 HRS. Methods. This study was carried out in a group of 92 patients with cirrhosis (44 patients with type-2 HRS and 48 without HRS) and 50 healthy controls. Polymorphisms were determined by polymerase chain reaction (PCR) and melting curve analysis. Results. We did not find any significant difference in allele and genotype distributions of the eNOS -T-786C polymorphism among the groups (p = 0.440). However, the frequency of GT (40.9%) and TT (13.6%) genotypes and mutant allele T (34.1%) for the eNOS G894T polymorphism were significantly higher (p < 0.001 and p < 0.001, resp.) in the HRS group than in both the stable cirrhosis (14.6%, 4.2%, and 11.5%, resp.) and the control (22.0%, 2.0%, and 13.0%, resp.) groups. Conclusion. The occurrence of mutant genotypes (GT/TT) and mutant allele T in eNOS -G894T polymorphisms should be considered as a potential risk factor in cirrhotic patients with HRS.

Highlights

Hepatorenal syndrome (HRS), one of the most severe complications of cirrhosis, is defined as functional renal failure in the course of an acute liver injury and/or chronic liver disease
We determined that endothelial nitric oxide synthase (eNOS) polymorphism for G894T is a risk factor in the development of hepatorenal syndrome (HRS), despite finding no relationship between the eNOS polymorphism for T-786C and HRS
This experiment is the first study on the relationship between eNOS gene polymorphisms and HRS

Summary

Introduction

Hepatorenal syndrome (HRS), one of the most severe complications of cirrhosis, is defined as functional renal failure in the course of an acute liver injury and/or chronic liver disease. Type-1 HRS is a form of rapidly progressing renal failure defined by a doubling of the baseline serum creatinine to a level greater than 2.5 mg/dL in less than two weeks. This study was carried out in a group of 92 patients with cirrhosis (44 patients with type-2 HRS and 48 without HRS) and 50 healthy controls. We did not find any significant difference in allele and genotype distributions of the eNOS -T-786C polymorphism among the groups (p = 0.440). The frequency of GT (40.9%) and TT (13.6%) genotypes and mutant allele T (34.1%) for the eNOS G894T polymorphism were significantly higher (p < 0.001 and p < 0.001, resp.) in the HRS group than in both the stable cirrhosis (14.6%, 4.2%, and 11.5%, resp.) and the control (22.0%, 2.0%, and 13.0%, resp.) groups. The occurrence of mutant genotypes (GT/TT) and mutant allele T in eNOS -G894T polymorphisms should be considered as a potential risk factor in cirrhotic patients with HRS

Objectives

Methods

Results

Conclusion