Association of death receptor 4 and death receptor 5 gene polymorphisms with ulcerative colitis susceptibility

Abstract

Objective To explore the association of death receptor (DR) 4 and DR5 gene polymorphisms with ulcerative colitis (UC) susceptibility.Methods A total of 362 UC patients and 490 healthy individuals were recruited.The genotype of three locus of DR4 (rs20575,rs13278062)and DR5 (rs1047266) were detected by mini-sequencing technique (SNaPshot).Chi-square test and Fisher's exact test were performed to compare the differences in the distribution of allele and genotypic frequencies.The association of DR4 and DR5 polymorphisms with clinical pathological characteristics of UC patients was analyzed by unconditional Logistic regression analysis.Results The frequencies of variant allele G and genotype CG + GG of DR4 (rs20575) in UC group were higher than those in healthy control group (3.18％ (23/724) vs 1.63％(16/980),x2=4.439,OR=1.977,95％CI:1.037 to3.769; P=0.035; 6.08％(22/362) vs3.27％(16/490),x2=3.863,OR 1.917,95％CI:0.992 to 3.705,P=0.049).And the frequencies of variant allele G and genotype CG+GG of DR4 (rs20575) of severe UC patients were higher than those of mild and moderate UC patients (8.00％(8/100) vs 2.40％ (15/624),OR-3.530,95％CI:1.456 to 8.560,P=0.008; 16.00％(8/50) vs 4.49％(14/312),OR=4.054,95％CI:1.605 to 10.243,P=0.004).There were no significant differences in the variant allele and genotypic frequencies of DR4 (rs13278062) between UC group and healthy control group (both P＞0.05).The frequencies of variant allele T and genotype GT+TT of DR4 (rs13278062) of severe UC patients were lower than those of mild and moderate UC patients (23.00％(23/100) vs 36.06％(225/624),OR=0.530,95％CI:0.323 to 0.868,P=0.011;42.00％(21/50) vs 61.22％(191/312),OR=0.459,95％CI:0.250 to 0.8410,P=0.010).There were no significant differences in the variant allele and genotypic frequencies of DR5 (rs1047266)between UC group and healthy control group (both P＞0.05).Conclusions The risk of suffering UC increases in mutant allele G of DR4 (rs20575) carriers.The mutation of DR4 (rs13278062) gene may influence the severity of UC.However,DR5 (rs1047266) gene polymorphism is not related to UC. Key words: Colitis, ulcerative; Apoptosis; Point mutation; Alleles; Polymorphism, genetic