Association of cytotoxic T lymphocyte 4 gene with auto-antibodies in children with type 1 diabetes

Abstract

Objective The aim of the study was to detect the association of CTLA4 gene with anti-insulin antibodies in type 1 diabetes in children. Background The objective was to evaluate the diagnostic workup done for diabetic children in its relation to clinical findings and diabetic control, and to study association of CTLA4 gene and anti-insulin antibodies in type 1 diabetes in children. Materials and methods The results showed no significant difference between male and females regarding serum anti-insulin antibodies and CTLA4 gene. There was a significant positive correlation between serum anti-insulin antibody and duration of disease of type 1 diabetes, but there was an insignificant association between CTLA4 gene and anti-insulin antibodies and type 1 diabetes. Results The study was carried out in the Pediatric Endocrinology and Genetic Units in Menoufia University Hospital and Damnhour Medical Institute. One hundred children with type 1 diabetes were enrolled (44 males and 56 females). Their age ranged from 2 to 17 years, with a mean of 9.3 ± 13.6 years. Data were collected including detailed history and thorough clinical examination, including anthropometric measures (body weight, standing height, BMI, and laboratory data). Assessments of serum anti-insulin antibodies and CTLA4 gene were done for all cases and for 100 apparently healthy control children (51 males and 49 females), whose age ranged from 3 to 16, with mean of 8.45 ± 3.48 years. Conclusion This study concluded that serum anti-insulin antibodies can be used as a marker in diagnosis of new cases of type 1 diabetes in children.