Association of cytokine gene polymorphisms with susceptibility to cutaneous leishmaniasis in a Turkish population.

Abstract

The objective of this study was to determine the association of TNF-α -308 G/A, IFN-γ +874T/A, IL-12B+1188 A/C, IL-10 -1082 G/A and IL-4 -590 C/T polymorphisms with susceptibility to CL. A total of 55 CL patients and 110 controls from Sanlıurfa province of Turkey were included to this study. Polymorphisms were genotyped by 'polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)' and 'amplification refractory mutation system-PCR (ARMS-PCR)' methods. A statistically significant difference was noted in the allele (P<.001, P=.002) and genotype (P<.001, P=.001,) frequencies of TNF-α -308 G/A and IL-4 -590 C/T, respectively. TNF-α 308 GG versus GA genotype (OR=19.556 [95% CI 8.310-46.019] P<.001), GG versus GA+AA genotype (OR=20.444 [95% CI 8.707-48.004] P<.001) and G versus A allele (OR=6.968 [95% CI 3.903-12.440] P<.001) revealed significant association with CL. IL-4 -590 CC versus TT+CT genotype (OR=2.049 [95% CI 1.025-4.096], P=.041) and C versus T allele (OR=2.441 [95% CI 1.355-4.396], P=.002) revealed significant association with CL. Our study indicates that TNF-α 308 G/A and IL-4-590 C/T polymorphisms are significantly associated with susceptibility to CL. Individuals carrying A allele at TNF-α promoter -308 position and T allele at IL-4 promoter -590 position are at a higher risk for CL.