Association of CYP1A2 Gene rs17861162 Polymorphism and Breast Cancer Risk in Iranian Women: A Case-control Study

Abstract

Background: Breast cancer is the most commonly diagnosed malignant neoplasm among women worldwide. The significance of gene polymorphism in cytochrome P450 (CYP) 1A2 (CYP3A4) in cancer development is generally acknowledged. Objectives: The objective of this study was to investigate the possible association between the rs17861162 polymorphism of the CYP1A2 gene and vulnerability to breast cancer among women of Iranian heritage. Methods: In the current study, a cohort comprising 100 women who were diagnosed with breast cancer was identified as the case group in a case-control inquiry. Another cohort of 100 women in good health and residing in East Azerbaijan province, with similar age and ethnicity attributes to the individuals in the case group, were selected as the control group. The genomic DNA was obtained from leukocytes isolated from peripheral blood through a phenol-chloroform extraction method. The genotyping experiment was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Results: The findings of this study indicated that genotypic distribution among individuals diagnosed with breast cancer was 54% CC, 37% CG, and 9% GG. On the other hand, the healthy control group exhibited a distribution of 69% CC, 27% CG, and 2% GG. The statistical analysis revealed notable variations in the genotype and allele frequencies of the rs17861162 polymorphism in the CYP1A2 gene between people diagnosed with breast cancer and healthy control subjects (P>0.05). Conclusions: The results of our study indicated a significant association between breast cancer and the rs17861162 polymorphism in the CYP1A2 gene among Iranian women. In order to gain a comprehensive understanding of the specific ramifications of this polymorphism concerning breast cancer prognosis, diagnosis, and risk, it is imperative to perform additional research that spans a wide range of ethnic and geographical populations.