Abstract
Proper wound healing is dynamic in order to maintain the corneal integrity and transparency. Impaired or delayed corneal epithelial wound healing is one of the most frequently observed ocular defect and difficult to treat. Cyclin dependen kinase (cdk), a known cell cycle regulator, required for proper proliferating and migration of cell. We therefore investigated the role of cell cycle regulator cdk10, member of cdk family and its functional association with transcriptional factor (ETS2) at active phase of corneal epithelial cell migration. Our data showed that cdk10 was associated with ETS2, while its expression was upregulated at the active phase (18 hours) of cell migration and gradually decrease as the wound was completely closed. Topical treatment with anti-cdk10 and ETS2 antibodies delayed the wound closure time at higest concentration (10 µg/ml) compared to control. Further, our results also showed increased mRNA expression of cdk10 and ETS2 at active phase of migration at approximately 2 fold. Collectively, our data reveals that cdk10 and ETS2 efficiently involved during corneal wound healing. Further studies are warranted to better understand the mechanism and safety of topical cdk10 and ETS2 proteins in corneal epithelial wound-healing and its potential role for human disease treatment.
Highlights
Proper wound healing is dynamic in order to maintain the corneal integrity and transparency
The extent of healing was defined as the ratio of the differences between the original and the residual wound area
We have found that exogenous anti-cdk[10] and anti-ETS2 antibodies retard corneal epithelial wound healing at both concentrations, they are most effective at higher concentration (10 ug/ml)
Summary
Proper wound healing is dynamic in order to maintain the corneal integrity and transparency. Impaired or delayed corneal epithelial wound healing is one of the most frequently observed ocular defect and difficult to treat. Cyclin dependen kinase (cdk), a known cell cycle regulator, required for proper proliferating and migration of cell. We investigated the role of cell cycle regulator cdk[10], member of cdk family and its functional association with transcriptional factor (ETS2) at active phase of corneal epithelial cell migration. Our data showed that cdk[10] was associated with ETS2, while its expression was upregulated at the active phase (18 hours) of cell migration and gradually decrease as the wound was completely closed. Further studies are warranted to better understand the mechanism and safety of topical cdk[10] and ETS2 proteins in corneal epithelial wound-healing and its potential role for human disease treatment. Cdk[10] is a Cdc2-related kinase, which was previously reffered as PISSLRE8,9, exerts a positive control on cell division and act as driving regulator during the G2 or M phase of the cell cycle
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